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[具有与Mu1噬菌体DNA结构相似的DNA结构的铜绿假单胞菌噬菌体。II. D3112、B3和B39噬菌体之间相似性的证据:限制性内切核酸酶切割DNA的分析、D3112和B3重组噬菌体的分离]

[Pseudomonas aeruginosa bacteriophages with DNA structure similar to the DNA structure of Mu1 phage. II. Evidence for similarity between D3112, B3, and B39 bacteriophages: analysis of DNA splits by restriction endonucleases, isolation of D3112 and B3 recombinant phages].

作者信息

Krylov V N, Bogush V G, Ianenko A S, Kirsanov N B

出版信息

Genetika. 1980;16(6):975-84.

PMID:6256259
Abstract

It is found that bacteriophages B3 and B39 specific for Pseudomonas aeruginosa have the same genome structure as previously described phage D3112. On the right (S) end of their genomes a variable non-phage DNA is located (approximately 0.9-2.5 kilobases for different phages). It is probable that this variable DNa has its origin from different regions of bacterial chromosome. In genome of one of the phages, B3 phage, such variable DNA (not more than 150 base pairs) was found on the left end of DNA molecule. Isolation of a viable B3XD3112 recombinant phage and analysis of its genome with restriction technique and with studies of homo- and heteroduplex molecules had confirmed genetical relationship of B3 and D3112. Some essential non-homology of B3 and D3112 DNAs have been found on the right ends of genomes of the phages.

摘要

已发现,对铜绿假单胞菌具有特异性的噬菌体B3和B39与先前描述的噬菌体D3112具有相同的基因组结构。在其基因组的右端(S),有一段可变的非噬菌体DNA(不同噬菌体约为0.9 - 2.5千碱基)。这段可变DNA可能起源于细菌染色体的不同区域。在其中一种噬菌体B3的基因组中,在DNA分子的左端发现了这样的可变DNA(不超过150个碱基对)。通过分离出一种有活力的B3XD3112重组噬菌体,并利用限制技术以及对同源和异源双链分子的研究对其基因组进行分析,证实了B3和D3112之间的遗传关系。在噬菌体基因组的右端发现了B3和D3112 DNA的一些基本非同源性。

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