通过肿瘤切片短期培养评估头颈部鳞状细胞癌对西妥昔单抗的个体敏感性。
Evaluation of individual sensitivity of head and neck squamous cell carcinoma to cetuximab by short-term culture of tumor slices.
作者信息
Peria Marine, Donnadieu Jérôme, Racz Caroline, Ikoli Jean-Fortuné, Galmiche Antoine, Chauffert Bruno, Page Cyril
机构信息
Department of Head and Neck Surgery, University Hospital, Amiens, France.
Department of Maxillofacial Surgery, University Hospital, Amiens, France.
出版信息
Head Neck. 2016 Apr;38 Suppl 1:E911-5. doi: 10.1002/hed.24126. Epub 2015 Aug 13.
BACKGROUND
Cetuximab is a targeted therapy with demonstrated efficacy in the management of head and neck squamous cell carcinoma (HNSCC). However, no laboratory assay is available to predict its efficacy in an individual patient.
METHODS
Short-term cultures of tumor slices were performed on 9 tumor samples obtained after surgical resection in patients. Cancer cell proliferation was evaluated by immunohistochemistry and the impact of cetuximab on cell proliferation was examined.
RESULTS
Tumor architecture and the heterogeneous composition of HNSCC were preserved for at least 48 hours during short-term culture of tumor slices. HNSCC cells demonstrated a heterogeneous individual response to cetuximab.
CONCLUSION
Short-term culture of tumor slices is a strategy to estimate the clinical activity of cetuximab in individual patients with HNSCC. Further studies are required to correlate the results obtained with the clinical response of individual patients to cetuximab. © 2015 Wiley Periodicals, Inc. Head Neck 38: E911-E915, 2016.
背景
西妥昔单抗是一种靶向疗法，在头颈部鳞状细胞癌（HNSCC）的治疗中已显示出疗效。然而，尚无实验室检测方法可预测其在个体患者中的疗效。
方法
对9例患者手术切除后获得的肿瘤样本进行肿瘤切片短期培养。通过免疫组织化学评估癌细胞增殖情况，并检测西妥昔单抗对细胞增殖的影响。
结果
在肿瘤切片短期培养过程中，HNSCC的肿瘤结构和异质性组成至少维持了48小时。HNSCC细胞对西妥昔单抗表现出异质性的个体反应。
结论
肿瘤切片短期培养是评估西妥昔单抗在个体HNSCC患者中临床活性的一种策略。需要进一步研究将所得结果与个体患者对西妥昔单抗的临床反应相关联。©2015威利期刊公司。《头颈》38: E911 - E915, 2016。
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