Eevers N, Gielen M, Sánchez-López A, Jaspers S, White J C, Vangronsveld J, Weyens N
Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, Diepenbeek, 3590, Belgium.
Edafología, Soil and Environmental Chemistry Lab, Colegio de Postgraduados, km 36.5 Carretera Mexico-Texcoco, Estado de Mexico, 56230, Mexico.
Microb Biotechnol. 2015 Jul;8(4):707-15. doi: 10.1111/1751-7915.12291. Epub 2015 May 20.
Many endophytes have beneficial effects on plants and can be exploited in biotechnological applications. Studies hypothesize that only 0.001-1% of all plant-associated bacteria are cultivable. Moreover, even after successful isolations, many endophytic bacteria often show reduced regrowth capacity. This research aimed to optimize isolation processes and culturing these bacteria afterwards. We compared several minimal and complex media in a screening. Beside the media themselves, two gelling agents and adding plant extract to media were investigated to enhance the number and diversity of endophytes as well as the growth capacity when regrown after isolation. In this work, 869 medium delivered the highest numbers of cultivable bacteria, as well as the highest diversity. When comparing gelling agents, no differences were observed in the numbers of bacteria. Adding plant extract to the media lead to a slight increase in diversity. However, when adding plant extract to improve the regrowth capacity, sharp increases of viable bacteria occurred in both rich and minimal media.
许多内生菌对植物具有有益作用，可用于生物技术应用。研究推测，所有与植物相关的细菌中只有0.001% - 1%是可培养的。此外，即使成功分离后，许多内生细菌的再生长能力往往也会降低。本研究旨在优化分离过程并随后培养这些细菌。我们在筛选中比较了几种基本培养基和复杂培养基。除了培养基本身，还研究了两种凝胶剂以及向培养基中添加植物提取物，以提高内生菌的数量和多样性以及分离后再生长时的生长能力。在这项工作中，869培养基培养出的可培养细菌数量最多，多样性也最高。比较凝胶剂时，细菌数量未观察到差异。向培养基中添加植物提取物导致多样性略有增加。然而，当添加植物提取物以提高再生长能力时，丰富培养基和基本培养基中的活菌数量均大幅增加。
