Zhao Yanli, Dong Hongwei, Chen Xiaoqing, Gao Chao, Liu Qiuyan, Yang Songtao, Hu Guixue
Bing Du Xue Bao. 2015 Jan;31(1):74-9.
Feline calicivirus (FCV) is an important and highly prevalent pathogen of cats that causes feline respiratory disease. The reverse genetic systems for FCV have been established in national and international laboratories since 1995. This technique has been used widely in FCV basic research and good progress has consequently been made to determine the relationship between viral genome structures and the function of their proteins, the expression of foreign proteins, virus-host interactions, and viral pathogenic mechanisms. In this article,we review the state of progress with regards to the establishment and application of the FCV reverse genetic operating system,which will provide a useful reference tool for future related research.
猫杯状病毒（FCV）是一种重要且高度流行的猫病原体，可引起猫呼吸道疾病。自1995年以来，国内外实验室已建立了FCV的反向遗传系统。该技术已广泛应用于FCV的基础研究，并在确定病毒基因组结构与其蛋白质功能之间的关系、外源蛋白质的表达、病毒与宿主的相互作用以及病毒致病机制等方面取得了良好进展。在本文中，我们综述了FCV反向遗传操作系统的建立和应用方面的进展情况，这将为未来的相关研究提供有用的参考工具。
