Sánchez Irais, Reynoso-Camacho Rosalia, Salgado Luis M
CICATA-IPN, CICATA-QRO, National Polytecnic Institute, Cerro Blanco No. 141, Col. Colinas del Cimatario CP, 76090, Queretaro, QRO, Mexico.
Genes Nutr. 2015 Jul;10(4):471. doi: 10.1007/s12263-015-0471-5. Epub 2015 May 22.
Consuming a high-fat/high-fructose diet (HFD) starting at a young age leads to the development of obesity and to the progression of metabolic syndrome (MS). We are interested in the relationship between MS and DNA methylation as a mediator of the metabolic memory and the early appearance of these diseases in the progeny. To this end, Wistar rats were fed a HFD for 1 year, and every 12 weeks, biochemical analyses were performed. After 24 weeks, animals fed the HFD showed alterations related to MS such as elevated blood levels of fasting glucose, triglycerides, and insulin compared with their littermate controls. During the experimental period, the control females exhibited a 40 % lower 5-methylcytosine (5-mC) level compared to the control males. The HFD affected the 5-mC levels in males and females differently. The HFD induced a 20 % decrease in the 5-mC levels in males and a 15 % increase in females. We found that the HFD induces an early presentation of MS in the progeny of treated animals and that the DNA methylation was altered in the F1 generation. The presentation of MS is positively associated with changes in the global percentage of 5-mC in the DNA.
从年轻时就开始食用高脂肪/高果糖饮食（HFD）会导致肥胖以及代谢综合征（MS）的进展。我们感兴趣的是MS与DNA甲基化之间的关系，DNA甲基化作为代谢记忆的介质以及这些疾病在后代中的早期出现。为此，给Wistar大鼠喂食HFD 1年，每12周进行一次生化分析。24周后，与同窝对照相比，喂食HFD的动物出现了与MS相关的改变，如空腹血糖、甘油三酯和胰岛素的血液水平升高。在实验期间，对照雌性的5-甲基胞嘧啶（5-mC）水平比对照雄性低40%。HFD对雄性和雌性的5-mC水平影响不同。HFD使雄性的5-mC水平降低20%，使雌性增加15%。我们发现，HFD会导致经处理动物的后代早期出现MS，并且F1代的DNA甲基化发生了改变。MS的出现与DNA中5-mC总体百分比的变化呈正相关。
