Prabhakar Pankaj, Reeta K H, Maulik Subir Kumar, Dinda Amit Kumar, Gupta Yogendra Kumar
a Department of Pharmacology, All India Institute of Medical Sciences, New Delhi-110029, India.
b Department of Pathology, All India Institute of Medical Sciences, New Delhi-110029, India.
Appl Physiol Nutr Metab. 2017 Jan;42(1):23-32. doi: 10.1139/apnm-2016-0088. Epub 2016 Sep 2.
This study investigated the effect of α-amyrin (a pentacyclic triterpene) on high-fructose diet (HFD)-induced metabolic syndrome in rats. Male Wistar rats were randomly distributed into different groups. The control group was fed normal rat chow diet. The HFD group was fed HFD (60%; w/w) for 42 days. Pioglitazone (10 mg/kg, orally, once daily) was used as a standard drug. α-Amyrin was administered in 3 doses (50, 100, and 200 mg/kg, orally, once daily along with HFD). Plasma glucose, total cholesterol, triglycerides, and high-density lipoprotein cholesterol (HDL-C) were estimated. Changes in blood pressure, oral glucose tolerance, and insulin tolerance were measured. Hepatic oxidative stress as well as messenger RNA (mRNA) and protein levels of peroxisome proliferator-activated receptor alpha (PPAR-α) were analyzed. A significant increase in systolic blood pressure, plasma glucose, total cholesterol, and plasma triglycerides and a significant decrease in HDL-C were observed in HFD rats as compared with control rats. Glucose tolerance and insulin tolerance were also significantly impaired with HFD. α-Amyrin prevented these changes in a dose-dependent manner. Hepatic oxidative stress as well as micro- and macrovesicular fatty changes in hepatocytes caused by HFD were also attenuated by α-amyrin. α-Amyrin preserved the hepatic mRNA and protein levels of PPAR-α, which was reduced in HFD group. This study thus demonstrates that α-amyrin attenuates HFD-induced metabolic syndrome in rats.
本研究调查了α-香树脂醇（一种五环三萜）对高果糖饮食（HFD）诱导的大鼠代谢综合征的影响。雄性Wistar大鼠被随机分为不同组。对照组喂食正常大鼠饲料。HFD组喂食HFD（60%；w/w）42天。吡格列酮（10mg/kg，口服，每日一次）用作标准药物。α-香树脂醇以3种剂量（50、100和200mg/kg，口服，与HFD一起每日一次）给药。测定血浆葡萄糖、总胆固醇、甘油三酯和高密度脂蛋白胆固醇（HDL-C）。测量血压、口服葡萄糖耐量和胰岛素耐量的变化。分析肝脏氧化应激以及过氧化物酶体增殖物激活受体α（PPAR-α）的信使核糖核酸（mRNA）和蛋白质水平。与对照大鼠相比，HFD大鼠的收缩压、血浆葡萄糖、总胆固醇和血浆甘油三酯显著升高，HDL-C显著降低。HFD还显著损害了葡萄糖耐量和胰岛素耐量。α-香树脂醇以剂量依赖的方式预防了这些变化。α-香树脂醇还减轻了HFD引起的肝脏氧化应激以及肝细胞中的微泡和大泡脂肪变化。α-香树脂醇保留了HFD组中降低的肝脏PPAR-α的mRNA和蛋白质水平。因此，本研究表明α-香树脂醇可减轻HFD诱导的大鼠代谢综合征。
