Wei Aili, Zhang Xuewu, Wei Dong, Chen Gu, Wu Qingyu, Yang Shang-Tian
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
J Ind Microbiol Biotechnol. 2009 Nov;36(11):1383-9. doi: 10.1007/s10295-009-0624-x. Epub 2009 Jul 25.
Heterotrophic fermentation of microalgae has been shown to accumulate high amounts of microalgal lipids, which are regarded as one of the most promising feedstocks for sustainable biodiesel production. To increase the biomass and reduce the cost of microalgal culture, the purpose of this study was to evaluate the possibility of using cassava starch hydrolysate (CSH) instead of glucose as carbon source for heterotrophic culture of Chlorella protothecoides in flasks. First, the two-step enzymatic process of hydrolysis of cassava starch by alpha-amylase and glucoamylase was optimized; the conversion efficiency for cassava starch was up to 97.7%, and over 80% of CSH was glucose. Subsequently, we compared heterotrophic cultures of C. protothecoiedes using glucose or CSH as carbon source. The results demonstrated that when using CSH as the organic carbon source, the highest biomass and the maximum total lipid yield obtained were 15.8 and 4.19 g/L, representing increases of 42.3 and 27.7%, respectively, compared to using glucose as the organic carbon source. This suggests that CSH is a better carbon source than glucose for heterotrophic Chlorella protothecoides.
微藻的异养发酵已被证明能积累大量的微藻脂质，这些脂质被视为可持续生物柴油生产中最有前景的原料之一。为了增加生物量并降低微藻培养成本，本研究的目的是评估使用木薯淀粉水解物（CSH）代替葡萄糖作为碳源，在摇瓶中对原球藻进行异养培养的可能性。首先，对α-淀粉酶和糖化酶两步酶解木薯淀粉的过程进行了优化；木薯淀粉的转化效率高达97.7%，且超过80%的CSH为葡萄糖。随后，我们比较了以葡萄糖或CSH作为碳源对原球藻进行异养培养的情况。结果表明，当使用CSH作为有机碳源时，获得的最高生物量和最大总脂质产量分别为15.8 g/L和4.19 g/L，与使用葡萄糖作为有机碳源相比，分别增加了42.3%和27.7%。这表明对于异养的原球藻来说，CSH是比葡萄糖更好的碳源。
