College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China.
College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China.
Carbohydr Polym. 2015 Jan 22;115:25-31. doi: 10.1016/j.carbpol.2014.08.082. Epub 2014 Sep 2.
The extraction process of polysaccharides from Rana chensinensis skin was optimized by using a Box-Behnken design. The optimum extraction conditions were as follows: extraction time, 4.96h; extraction temperature, 100°C; ratio of water to raw material, 60; and extraction frequency, 1. Under these conditions, the experimental polysaccharide yield was 2.03±0.14%, which agreed with the predicted yield. The purified polysaccharide RCSP II was successfully obtained by diethylaminoethanol-Sepharose and Sepharose CL-6B column chromatography. In vitro experiments showed that RCSP II exhibited a strong scavenging activity against superoxide anion and 1,1-diphenyl-2-picrylhydrazyl radicals but a weak scavenging activity against hydroxyl radicals. RCSP II also showed a strong reducing capacity. Thus, this polysaccharide can be used as a natural antioxidant in functional foods or medicines.
采用 Box-Behnken 设计优化了中华大蟾蜍皮肤多糖的提取工艺。最佳提取条件为：提取时间 4.96h、提取温度 100°C、料液比 60、提取次数 1。在此条件下，实验多糖得率为 2.03±0.14%，与预测值相符。采用二乙氨基乙醇 - 琼脂糖和琼脂糖 CL-6B 柱色谱法成功获得纯化多糖 RCSP II。体外实验表明，RCSP II 对超氧阴离子自由基和 1,1-二苯基-2-苦基肼自由基具有较强的清除活性，对羟自由基的清除活性较弱。RCSP II 还具有较强的还原能力。因此，该多糖可作为功能性食品或药物中的天然抗氧化剂。
