Federal University of Rio de Janeiro, Chemistry Institute, CT, Bloco A, Av. Athos da Silveira Ramos, 149, Rio de Janeiro CEP 21941-909, RJ, Brazil.
Federal University of Rio de Janeiro, Chemistry Institute, CT, Bloco A, Av. Athos da Silveira Ramos, 149, Rio de Janeiro CEP 21941-909, RJ, Brazil; National Institute of Technology, Ministry of Science, Technology and Innovation, Avenida Venezuela, 82, Rio de Janeiro CEP 20081-312, RJ, Brazil.
Carbohydr Polym. 2015 Sep 5;128:75-81. doi: 10.1016/j.carbpol.2015.03.087. Epub 2015 Apr 7.
Cellulose nanocrystals (CNCs), a biomaterial with high added value, were obtained from pure cellulose, Eucalyptus holocellulose, unbleached Kraft pulp, and sugarcane bagasse, by fibrillating these biomass substrates using wet disk milling (WDM) followed by enzymatic hydrolysis using endoglucanase/β-glucosidase. The hydrolysis experiments were conducted using the commercial enzyme OptimashBG or a blend of Pyrococcus horikoshii endoglucanase and Pyrococcus furiosus β-glucosidase. The fibrillated materials and CNCs were analyzed by X-ray diffraction, atomic force microscopy, scanning electron microscopy, and the specific surface area (SSA) was measured. WDM resulted in the formation of long and twisted microfibers of 1000-5000 nm in length and 4-35 nm in diameter, which were hydrolyzed into shorter and straighter CNCs of 500-1500 nm in length and 4-12 nm in diameter, with high cellulose crystallinity. Therefore, the CNC's aspect ratio was successfully adjusted by endoglucanases under mild reaction conditions, relative to the reported acidic hydrolysis method.
纤维素纳米晶体（CNCs）是一种具有高附加值的生物材料，可通过湿盘式磨浆（WDM）将纯纤维素、桉木全纤维素、未漂硫酸盐浆和甘蔗渣等生物质基质纤维化，然后使用内切葡聚糖酶/β-葡萄糖苷酶进行酶水解来获得。水解实验使用商业酶OptimashBG 或 Pyrococcus horikoshii 内切葡聚糖酶和 Pyrococcus furiosus β-葡萄糖苷酶的混合物进行。通过 X 射线衍射、原子力显微镜、扫描电子显微镜和比表面积（SSA）测量对原纤化材料和 CNC 进行了分析。WDM 形成了长而扭曲的微纤维，长度为 1000-5000nm，直径为 4-35nm，这些微纤维被水解成长度为 500-1500nm、直径为 4-12nm 的更短、更直的 CNC，具有高纤维素结晶度。因此，与报道的酸性水解方法相比，内切葡聚糖酶在温和的反应条件下成功地调节了 CNC 的纵横比。
