Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University (Formerly University of Pune), Pune 411007, India.
Toxoid Purification Department, Serum Institute of India Ltd., Hadapsar, Pune 411028, India.
Carbohydr Polym. 2015 Sep 5;128:188-98. doi: 10.1016/j.carbpol.2015.04.020. Epub 2015 Apr 21.
Microspheres were prepared from water soluble chitosan using dual vanillin/TPP crosslinkers. Placebo (C1), Bovine serum albumin (BSA) (C2), monovalent tetanus toxoid (TT) (C3) and divalent tetanus (TT) and diphtheria toxoids (DT) (C4) encapsulated microspheres were studied in terms of size (1-4 μm), encapsulation efficiency (75-80%), swelling and mucoadhesion (56-68%). FT-IR, TGA, XRD and SEM characterization of microspheres suggested specific interaction, more thermal stability, amorphous nature and rough surfaces of encapsulated microspheres. EDS confirmed the co-crosslinking and ninhydrin tests were showing higher crosslinking density. Zeta potential was 47.7 to 66.2 +mV indicating the potential stability of the colloidal system. Equilibrium adsorption isotherms described encapsulated microspheres followed the Langmuir isotherm model, suggesting monolayer adsorption of the mucin on microspheres. In-vitro release studies up to four weeks indicated zero order kinetics and obeyed swelling-controlled super case II transport release mechanism. Thus, the present study could be helpful in developing the multivalent oral vaccine.
采用双重香草醛/TPP 交联剂从水溶性壳聚糖中制备微球。研究了安慰剂（C1）、牛血清白蛋白（BSA）（C2）、单价破伤风类毒素（TT）（C3）和双价破伤风和白喉类毒素（DT）（C4）包封微球的粒径（1-4μm）、包封效率（75-80%）、溶胀和粘膜粘附（56-68%）。FT-IR、TGA、XRD 和 SEM 对微球的特征分析表明，包封微球具有特定的相互作用、更高的热稳定性、无定形性质和粗糙的表面。EDS 证实了共聚交联，而茚三酮试验表明交联密度更高。Zeta 电位为 47.7 至 66.2+mV，表明胶体系统具有潜在的稳定性。平衡吸附等温线表明，包封微球遵循 Langmuir 等温线模型，表明粘蛋白在微球上的单层吸附。在长达四周的体外释放研究中，零级动力学表明，该释放机制遵循溶胀控制的超二级转运释放机制。因此，本研究可能有助于开发多价口服疫苗。
