水刀辅助吸脂术获取的人脂肪间充质干细胞的分离及分化潜能
Isolation and differentiation potential of human mesenchymal stem cells from adipose tissue harvested by water jet-assisted liposuction.
作者信息
Meyer Juliane, Salamon Achim, Herzmann Nicole, Adam Stefanie, Kleine Hans-Dieter, Matthiesen Inge, Ueberreiter Klaus, Peters Kirsten
机构信息
Mrs Meyer and Mrs Herzmann are PhD Students, Dr Salamon is a Post-doctoral Fellow, Mrs Adam is a Technical Assistant, and Dr Peters is Head of the Stem Cell Biology Group, Department of Cell Biology, Rostock University Medical Center, Rostock, Germany. Dr Kleine is on the Executive Board of Seracell Stammzelltechnologie GmbH, Rostock, Germany. Dr Matthiesen is Head of the Department of Medical Affairs, human med AG, Schwerin, Germany. Dr Ueberreiter is a Plastic Surgeon in private practice in Birkenwerder, Germany.
出版信息
Aesthet Surg J. 2015 Nov;35(8):1030-9. doi: 10.1093/asj/sjv075. Epub 2015 May 24.
BACKGROUND
In recent years the therapeutic application of extracted adipose tissue for autologous fat grafting and the application of adipose tissue-derived mesenchymal stem cells (adMSC) isolated thereof has progressed. Water-jet assisted liposuction (WAL) is 1 procedure for harvesting adipose tissue and provides a favorable aesthetic outcome combined with high tissue protection. Tissue aspirated by WAL has been successfully applied in grafting procedures.
OBJECTIVES
The aims of this study were to confirm the tissue viability and to understand the abundance and mesenchymal differentiation capacity of stem cells within the tissue.
METHODS
We analyzed tissue integrity of WAL tissue particles via fluorescence microscopy. The adMSC content was determined by isolating the cells from the tissue. The mesenchymal differentiation capacity was confirmed with cytochemical staining methods.
RESULTS
The stromal vascular fraction of WAL tissue showed high viability and contained an average of 2.6 × 105 CD34-positive cells per milliliter of tissue. Thus WAL tissue contains a high number of stem cells. Furthermore adMSC isolated from WAL tissue showed typical mesenchymal differentiation potential.
CONCLUSIONS
WAL of adipose tissue is well suited for autologous fat grafting because it retains tissue viability. Furthermore it is a valid source for the subsequent isolation of adMSC with multipotent differentiation potential.
LEVEL OF EVIDENCE
3 Therapeutic.
背景
近年来，提取的脂肪组织用于自体脂肪移植的治疗应用以及从中分离出的脂肪组织来源间充质干细胞（adMSC）的应用取得了进展。水刀辅助吸脂术（WAL）是一种获取脂肪组织的方法，与高度的组织保护相结合，能提供良好的美学效果。通过WAL抽吸的组织已成功应用于移植手术。
目的
本研究的目的是确认组织活力，并了解组织内干细胞的数量和间充质分化能力。
方法
我们通过荧光显微镜分析了WAL组织颗粒的组织完整性。通过从组织中分离细胞来确定adMSC含量。用细胞化学染色方法确认间充质分化能力。
结果
WAL组织的基质血管成分显示出高活力，每毫升组织平均含有2.6×10⁵个CD34阳性细胞。因此，WAL组织含有大量干细胞。此外，从WAL组织中分离出的adMSC显示出典型的间充质分化潜能。
结论
脂肪组织的WAL非常适合自体脂肪移植，因为它能保持组织活力。此外，它是随后分离具有多能分化潜能的adMSC的有效来源。
证据水平
3 治疗性。
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