Drobek Christoph, Meyer Juliane, Mau Robert, Wolff Anne, Peters Kirsten, Seitz Hermann
Chair of Microfluidics, University of Rostock, 18059 Rostock, Germany.
Department of Cell Biology, Rostock University Medical Center, 18057 Rostock, Germany.
iScience. 2022 Dec 10;26(1):105796. doi: 10.1016/j.isci.2022.105796. eCollection 2023 Jan 20.
To use regeneratively active cells for cell therapeutic applications, the cells must be isolated from their resident tissues. Different isolation procedures subject these cells to varying degrees of mechanical strain, which can affect the yield of cell number and viability. Knowledge of cell volumetric mass density is important for experimental and numerical optimization of these procedures. Although methods for measuring cell volumetric mass density already exist, they either consume much time and cell material or require a special setup. Therefore, we developed a user-friendly method that is based on the use of readily available instrumentation. The newly developed method is predicated on the linear relationship between the volumetric mass density of the cell suspension and the volumetric mass density, number, and diameter of the cells in the suspension. We used this method to determine the volumetric mass density of mesenchymal stem cells (MSCs) and compared it to results from the established density centrifugation.
为了将具有再生活性的细胞用于细胞治疗应用，必须从其驻留组织中分离出这些细胞。不同的分离程序会使这些细胞受到不同程度的机械应变，这可能会影响细胞数量和活力的产量。了解细胞体积质量密度对于这些程序的实验和数值优化很重要。尽管已经存在测量细胞体积质量密度的方法，但它们要么消耗大量时间和细胞材料，要么需要特殊设置。因此，我们开发了一种基于使用现成仪器的用户友好方法。新开发的方法基于细胞悬浮液的体积质量密度与悬浮液中细胞的体积质量密度、数量和直径之间的线性关系。我们使用这种方法来确定间充质干细胞（MSC）的体积质量密度，并将其与既定密度离心法的结果进行比较。
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