Mand Mario, Hahn Olga, Meyer Juliane, Peters Kirsten, Seitz Hermann
Chair of Microfluidics, Faculty of Mechanical Engineering and Marine Technology, University of Rostock, 18059 Rostock, Germany.
Institute of Cell Biology, Rostock University Medical Center, 18057 Rostock, Germany.
Bioengineering (Basel). 2024 Oct 11;11(10):1011. doi: 10.3390/bioengineering11101011.
Within the healthy human body, cells reside within the physiological environment of a tissue compound. Here, they are subject to constant low levels of mechanical stress that can influence the growth and differentiation of the cells. The liposuction of adipose tissue and the subsequent isolation of mesenchymal stem/stromal cells (MSCs), for example, are procedures that induce a high level of mechanical shear stress. As MSCs play a central role in tissue regeneration by migrating into regenerating areas and driving regeneration through proliferation and tissue-specific differentiation, they are increasingly used in therapeutic applications. Consequently, there is a strong interest in investigating the effects of shear stress on MSCs. In this study, we present a set-up for applying high shear rates to cells based on a rotational rheometer with a small-angle cone-plate configuration. This set-up was used to investigate the effect of various shear stresses on human adipose-derived MSCs in suspension. The results of the study show that the viability of the cells remained unaffected up to 18.38 Pa for an exposure time of 5 min. However, it was observed that intense shear stress damaged the cells, with longer treatment durations increasing the percentage of cell debris.
在健康人体中，细胞存在于组织复合物的生理环境中。在此环境下，它们会受到持续的低水平机械应力影响，而这种应力会影响细胞的生长和分化。例如，抽脂获取脂肪组织并随后分离间充质干/基质细胞（MSCs）的过程，会引发高水平的机械剪切应力。由于MSCs通过迁移到再生区域并通过增殖和组织特异性分化来驱动再生，在组织再生中发挥核心作用，因此它们越来越多地被用于治疗应用。因此，人们对研究剪切应力对MSCs的影响有着浓厚兴趣。在本研究中，我们展示了一种基于具有小角度锥板配置的旋转流变仪，用于对细胞施加高剪切速率的装置。该装置用于研究各种剪切应力对悬浮状态下的人脂肪来源MSCs的影响。研究结果表明，在暴露时间为5分钟的情况下，细胞活力在高达18.38 Pa时仍不受影响。然而，观察到强烈的剪切应力会损伤细胞，处理时间越长，细胞碎片的百分比越高。
