皮肤接触护理与早产儿口腔微生物群的发育
Skin-to-Skin Care and the Development of the Preterm Infant Oral Microbiome.
作者信息
Hendricks-Muñoz Karen D, Xu Jie, Parikh Hardik I, Xu Ping, Fettweis Jennifer M, Kim Yang, Louie Moi, Buck Gregory A, Thacker Leroy R, Sheth Nihar U
机构信息
Division of Neonatal Medicine, Department of Pediatrics, Virginia Commonwealth University School of Medicine, Richmond, Virginia.
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia.
出版信息
Am J Perinatol. 2015 Nov;32(13):1205-16. doi: 10.1055/s-0035-1552941. Epub 2015 May 22.
OBJECTIVE
The oral cavity represents an initial entry way for oral and gut indigenous colonization. Skin-to-skin (STS) care, in which the mother holds the diaper clad naked preterm (PT) infant between her breasts, is associated with improved digestive function, decreased stress, and improved survival. This study evaluated the development of oral microbial colonization repertoires and health characteristics in PT infants with or without STS exposure.
METHODS
Saliva from 42 PT infants (<32 weeks of gestation at birth) was collected prospectively at 1 month and/or at discharge. High-throughput 16S rRNA sequencing identified microbial diversity and prevalence of bacterial signatures correlated with clinical STS or non-STS care.
RESULTS
Corrected for gestational age (CGA) at sampling, bacterial taxa demonstrated increased Streptococcus as a signature of oral repertoire maturation. STS was associated with increased Streptococcus (p < 0.024), while non-STS was associated with greater Corynebacterium (p < 0.023) and Pseudomonas (p < 0.019) in infants ≤ 32 weeks CGA. In infants > 32 weeks CGA, Neisseria and Acinetobacter were more prevalent, 50 vs. 16.7% and 40 vs. 0%, respectively. STS care was associated with shorter hospitalization (p < 0.039).
CONCLUSION
STS care during earlier gestation was associated with a distinct microbial pattern and an accelerated pace of oral microbial repertoire maturity.
目的
口腔是口腔和肠道原生菌定植的初始入口途径。皮肤对皮肤（STS）护理，即母亲将穿着尿布的裸体早产（PT）婴儿抱在双乳之间，与消化功能改善、压力减轻和存活率提高有关。本研究评估了有无接受STS护理的PT婴儿口腔微生物定植谱的发育情况和健康特征。
方法
前瞻性收集42例PT婴儿（出生时孕周<32周）在1个月和/或出院时的唾液。高通量16S rRNA测序确定了与临床STS或非STS护理相关的微生物多样性和细菌特征的流行情况。
结果
校正采样时的孕周（CGA）后，细菌类群显示链球菌增加，这是口腔菌谱成熟的标志。在CGA≤32周的婴儿中，STS与链球菌增加有关（p<0.024），而非STS与棒状杆菌（p<0.023）和假单胞菌（p<0.019）增加有关。在CGA>32周的婴儿中，奈瑟菌和不动杆菌更为普遍，分别为50%对16.7%和40%对0%。STS护理与住院时间缩短有关（p<0.039）。
结论
妊娠早期的STS护理与独特的微生物模式和口腔微生物菌谱成熟加快有关。
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