Ahn T I, Jeon K W
Department of Zoology, University of Tennessee, Knoxville 37996.
J Protozool. 1989 Nov-Dec;36(6):560-2. doi: 10.1111/j.1550-7408.1989.tb01096.x.
The reactivity of a monoclonal antibody against actin of Amoeba proteus with actins from other sources was examined. The monoclonal antibody cross-reacted with actins from vertebrate muscles, human erythrocytes, and Acanthamoeba castellanii, but it did not react with Naegleria gruberi actin. The amoeba actin was resolved into 3 bands with isoelectric points of 5.96, 6.03 and 6.10 in electrofocusing gels and they corresponded to 3 peptide spots reacting with the antibody on 2-dimensional immunoblots.
检测了一种针对变形虫肌动蛋白的单克隆抗体与其他来源肌动蛋白的反应性。该单克隆抗体与来自脊椎动物肌肉、人类红细胞和卡氏棘阿米巴的肌动蛋白发生交叉反应，但不与格氏耐格里阿米巴肌动蛋白反应。在等电聚焦凝胶中，变形虫肌动蛋白被分离成3条带，其等电点分别为5.96、6.03和6.10，它们在二维免疫印迹上对应于与该抗体反应的3个肽斑点。
