微流控技术作为一种评估血栓形成和止血的新兴临床工具。
Microfluidic technology as an emerging clinical tool to evaluate thrombosis and hemostasis.
作者信息
Branchford Brian R, Ng Christopher J, Neeves Keith B, Di Paola Jorge
机构信息
Dept. of Pediatrics - Hematology/Oncology, University of Colorado School of Medicine, Aurora, CO, USA; University of Colorado Hemophilia and Thrombosis Center, Aurora, CO, USA.
Dept. of Chemical & Biological Engineering, Colorado School of Mines, Golden, CO, USA.
出版信息
Thromb Res. 2015 Jul;136(1):13-9. doi: 10.1016/j.thromres.2015.05.012. Epub 2015 May 21.
Abstract
Assessment of platelet function and coagulation under flow conditions can augment traditional static assays used to evaluate patients with suspected hemostatic or thrombotic disorders. Among the available flow-based assays, microfluidic devices require the smallest blood volume and provide multiple output options. These assays are based on the presence of wall shear stress that mimics in vivo interactions between blood components and vessel walls. Microfluidic devices can generate essential information regarding homeostatic regulation of platelet activation and subsequent engagement of the coagulation cascade leading to fibrin deposition and clot formation. Emerging data suggest that microfluidic assays may also reveal consistent patterns of hemostatic or thrombotic pathology, and could aid in assessing and monitoring patient-specific effects of coagulation-modifying therapies.
摘要
在流动条件下评估血小板功能和凝血功能，可以补充用于评估疑似止血或血栓形成障碍患者的传统静态检测方法。在现有的基于流动的检测方法中，微流控装置所需血量最少，并提供多种输出选项。这些检测方法基于壁面剪应力的存在，该剪应力模拟了血液成分与血管壁之间的体内相互作用。微流控装置可以生成有关血小板激活的稳态调节以及随后凝血级联反应的参与情况的重要信息，凝血级联反应会导致纤维蛋白沉积和凝块形成。新出现的数据表明，微流控检测方法还可能揭示止血或血栓形成病理的一致模式，并有助于评估和监测凝血调节疗法对特定患者的影响。
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Nat Nanotechnol. 2014 Dec;9(12):1047-53. doi: 10.1038/nnano.2014.250. Epub 2014 Nov 2.
2
Global assays of hemostasis.全球止血检测
Curr Opin Hematol. 2014 Sep;21(5):395-403. doi: 10.1097/MOH.0000000000000074.
3
Identification of platelet function defects by multi-parameter assessment of thrombus formation.通过血栓形成的多参数评估识别血小板功能缺陷。
Nat Commun. 2014 Jul 16;5:4257. doi: 10.1038/ncomms5257.
4
Optimal fluid resuscitation in trauma: type, timing, and total.创伤中的最佳液体复苏：类型、时机及总量
Curr Opin Crit Care. 2014 Aug;20(4):366-72. doi: 10.1097/MCC.0000000000000104.
5
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PLoS One. 2014 Jan 3;9(1):e82493. doi: 10.1371/journal.pone.0082493. eCollection 2014.
6
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Thromb Res. 2014 Feb;133(2):203-10. doi: 10.1016/j.thromres.2013.10.043. Epub 2013 Nov 6.
7
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Arterioscler Thromb Vasc Biol. 2014 Feb;34(2):397-407. doi: 10.1161/ATVBAHA.113.302547. Epub 2013 Dec 19.
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J Thromb Haemost. 2014;12(3):418-20. doi: 10.1111/jth.12482.
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10
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