Department of Biomedical Engineering, Texas A&M College of Engineering, College Station, TX, USA.
Division of Transfusion Medicine & Coagulation, Department of Pathology & Immunology, Texas Children's Hospital & Baylor College of Medicine, Houston, TX, USA.
Sci Rep. 2020 Apr 1;10(1):5742. doi: 10.1038/s41598-020-62768-4.
Accurate assessment of blood thrombosis and antithrombotic therapy is essential for the management of patients in a variety of clinical conditions, including surgery and on extracorporeal life support. However, current monitoring devices do not measure the effects of hemodynamic forces that contribute significantly to coagulation, platelet function and fibrin formation. This limits the extent to which current assays can predict clotting status in patients. Here, we demonstrate that a biomimetic microfluidic device consisting stenosed and tortuous arteriolar vessels would analyze blood clotting under flow, while requiring a small blood volume. When the device is connected to an inline pressure sensor a clotting time analysis is applied, allowing for the accurate measurement of coagulation, platelets and fibrin content. Furthermore, this device detects a prolonged clotting time in clinical blood samples drawn from pediatric patients on extracorporeal membrane oxygenation receiving anticoagulant therapy. Thus, this tortuosity activated microfluidic device could lead to a more quantitative and rapid assessment of clotting disorders and their treatment.
准确评估血液血栓形成和抗血栓治疗对于各种临床情况下的患者管理至关重要，包括手术和体外生命支持。然而，目前的监测设备无法测量对凝血、血小板功能和纤维蛋白形成有重大影响的血流动力。这限制了当前检测方法在预测患者凝血状态方面的程度。在这里，我们展示了一种由狭窄和扭曲的小动脉组成的仿生微流控设备，它可以在流动下分析血液凝固，同时需要少量血液。当设备与在线压力传感器连接时，应用凝血时间分析，可准确测量凝血、血小板和纤维蛋白含量。此外，该设备检测到接受抗凝治疗的体外膜氧合儿科患者的临床血液样本凝血时间延长。因此，这种迂曲激活微流控装置可实现对凝血障碍及其治疗的更定量和快速评估。
