Gilani S H, Fallon E, Kozam G
Department of Anatomy, New Jersey Medical School, Newark 07103.
Life Sci. 1989;45(22):2157-61. doi: 10.1016/0024-3205(89)90082-9.
The effects of minoxidil were studied on chick embryos of 24 and 48 hours of incubation. Minoxidil (3%) was injected into the air sacs of the eggs at doses of 20, 30, 40, and 50 microliters per egg. The controls received 100 microliters of physiological saline. All the embryos, including controls, were examined at day 13. The total number of eggs used in this study was 300. At 24 hours incubation, the percentage of survival ranged from 87 to 21 as the dosages of minoxidil were increased from 20 microliters to 50 microliters per egg (controls = 87%). The survival of the embryos ranged from 79% to 9% after the 48-hour treatment with the similar dosages of minoxidil utilized for the 24-hour group (controls = 83%). A low incidence of gross malformations such as twisted limbs, abnormal beak, short neck and everted viscera were observed; however, the increased incidence was not statistically significant when compared to controls. Body hemorrhage and edema were of high occurrence among the treated embryos. These effects are probably secondary to the known pharmacological effects of minoxidil. The frequency and types of gross malformations did not vary much in the 24 or 48-hour treated groups.
研究了米诺地尔对孵化24小时和48小时的鸡胚胎的影响。将3%的米诺地尔以每枚鸡蛋20、30、40和50微升的剂量注入气囊。对照组接受100微升生理盐水。在第13天对所有胚胎（包括对照组）进行检查。本研究使用的鸡蛋总数为300枚。在孵化24小时时，随着米诺地尔剂量从每枚鸡蛋20微升增加到50微升，存活率从87%降至21%（对照组为87%）。用与24小时组相同剂量的米诺地尔处理48小时后，胚胎存活率从79%降至9%（对照组为83%）。观察到肢体扭曲、喙异常、颈部短和内脏外翻等严重畸形的发生率较低；然而，与对照组相比，发生率的增加没有统计学意义。治疗后的胚胎中身体出血和水肿发生率较高。这些影响可能是米诺地尔已知药理作用的继发效应。在24小时或48小时治疗组中，严重畸形的频率和类型变化不大。
