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盘基网柄菌中控制早期基因表达诱导的顺式作用元件的鉴定。

Identification of a cis-acting element controlling induction of early gene expression in Dictyostelium discoideum.

作者信息

May T, Kern H, Müller-Taubenberger A, Nellen W

机构信息

Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.

出版信息

Mol Cell Biol. 1989 Nov;9(11):4653-9. doi: 10.1128/mcb.9.11.4653-4659.1989.

DOI:10.1128/mcb.9.11.4653-4659.1989
PMID:2601692
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363611/
Abstract

Using a new promoter analysis transformation vector for Dictyostelium discoideum (PAV-CAT), we have defined cis-acting elements in the promoter of the cyclic AMP-induced early-expressed gene A11H2, which encodes an alpha-fucosidase-related protein (A. Müller-Taubenberger, M. Westphal, A. Noegel, and G. Gerisch, FEBS Lett. 246:185-192, 1989). Sequences responsible for developmentally regulated gene induction could be separated from the basal promoter that conferred low levels of transcriptional activity. By gel shift experiments, we present evidence that the cis-acting element is the target of a trans-acting factor that by itself is subject to developmental regulation.

摘要

利用一种用于盘基网柄菌的新型启动子分析转化载体（PAV-CAT），我们确定了环磷酸腺苷诱导的早期表达基因A11H2启动子中的顺式作用元件，该基因编码一种与α-岩藻糖苷酶相关的蛋白质（A. Müller-Taubenberger、M. Westphal、A. Noegel和G. Gerisch，《欧洲生物化学学会联合会快报》246:185 - 192，1989年）。负责发育调控基因诱导的序列可以与赋予低水平转录活性的基础启动子分开。通过凝胶迁移实验，我们提供证据表明顺式作用元件是一种反式作用因子的靶标，而该反式作用因子自身也受发育调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea02/363611/b6e0fd2cf657/molcellb00059-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea02/363611/263937215bee/molcellb00059-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea02/363611/9f8baf745ebb/molcellb00059-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea02/363611/b6e0fd2cf657/molcellb00059-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea02/363611/263937215bee/molcellb00059-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea02/363611/9f8baf745ebb/molcellb00059-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea02/363611/b6e0fd2cf657/molcellb00059-0082-a.jpg

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本文引用的文献

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Identification of a DNA sequence element required for efficient expression of a developmentally regulated and cAMP-inducible gene of Dictyostelium discoideum.鉴定一个 DNA 序列元件，该元件是秀丽隐杆线虫中发育调控和 cAMP 诱导基因高效表达所必需的。
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Purification of the sequence-specific transcription factor CTCBF, involved in the control of human collagen IV genes: subunits with homology to Ku antigen.参与人类IV型胶原基因调控的序列特异性转录因子CTCBF的纯化：与Ku抗原具有同源性的亚基
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Constitutive overexpression of the contact site A glycoprotein enables growth-phase cells of Dictyostelium discoideum to aggregate.接触位点A糖蛋白的组成型过表达使盘基网柄菌的生长阶段细胞能够聚集。
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Two separable promoters control different aspects of expression of a Dictyostelium gene.两个可分离的启动子控制盘基网柄菌属基因表达的不同方面。
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New nucleotide sequence data on the EMBL File Server.欧洲分子生物学实验室文件服务器上的新核苷酸序列数据。
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Evidence for a feedback regulated back-up promoter which controls permanent expression of a Dictyostelium gene.关于一个反馈调节备用启动子的证据，该启动子控制盘基网柄菌基因的永久表达。
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Regulation of the Discoidin I gamma gene in Dictyostelium discoideum: identification of individual promoter elements mediating induction of transcription and repression by cyclic AMP.盘基网柄菌中盘状结构域蛋白Iγ基因的调控：介导转录诱导和环磷酸腺苷抑制的单个启动子元件的鉴定
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The cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum contains three promoters specific for growth, aggregation, and late development.盘基网柄菌的环核苷酸磷酸二酯酶基因包含三个分别在生长、聚集和发育后期特异的启动子。
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The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation.具有多用途克隆位点的pIC质粒和噬菌体载体，用于通过插入失活进行重组体筛选。
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