Pavlovic J, Haribabu B, Dottin R P
Institute for Biomolecular Structure and Function, Hunter College, City University of New York, New York 10021.
Mol Cell Biol. 1989 Nov;9(11):4660-9. doi: 10.1128/mcb.9.11.4660-4669.1989.
The signal transduction pathways that lead to gene induction are being intensively investigated in Dictyostelium discoideum. We have identified by deletion and transformation analysis a sequence element necessary for induction of a gene coding for uridine diphosphoglucose pyrophosphorylase (UDPGP1) of D. discoideum in response to extracellular cyclic AMP (cAMP). This regulatory element is located 380 base pairs upstream of the transcription start site and contains a G+C-rich partially palindromic sequence. It is not required for transcription per se but is required for induction of the gene in response to the stimulus of extracellular cAMP. The cAMP response sequence is also required for induction of the gene during normal development. A second A+T-rich cis-acting region located immediately downstream of the cAMP response sequence appears to be essential for the basal level of expression of the UDPGP1 gene. The position of the cAMP response element coincides with a DNase I-hypersensitive site that is observed when the UDPGP1 gene is actively transcribed.
在盘基网柄菌中，导致基因诱导的信号转导途径正在被深入研究。我们通过缺失和转化分析鉴定出了一个序列元件，它是盘基网柄菌中编码尿苷二磷酸葡萄糖焦磷酸化酶（UDPGP1）的基因响应细胞外环磷酸腺苷（cAMP）诱导所必需的。这个调控元件位于转录起始位点上游380个碱基对处，包含一个富含G + C的部分回文序列。它本身并非转录所必需，但对于基因响应细胞外cAMP刺激的诱导是必需的。在正常发育过程中，该基因的诱导也需要cAMP反应序列。位于cAMP反应序列紧邻下游的第二个富含A + T的顺式作用区域，似乎对于UDPGP1基因的基础表达水平至关重要。cAMP反应元件的位置与当UDPGP1基因被积极转录时观察到的一个DNase I超敏位点相重合。
