Del Ry Silvia, Cabiati Manuela, Bianchi Vanessa, Caponi Laura, Di Cecco Pietro, Marchi Benedetta, Randazzo Emioli, Caselli Chiara, Prescimone Tommaso, Clerico Aldo, Federico Giovanni
Laboratorio di Biochimica e Biologia Molecolare, CNR Institute of Clinical Physiology, Via Giuseppe Moruzzi 1, 56124, Pisa, Italy.
Sezione di Diabetologia Pediatrica, U.O. Pediatria Universitaria, Azienda Ospedaliero Universitaria Pisana, Pisa, Italy.
Eur J Nutr. 2016 Apr;55(3):1255-60. doi: 10.1007/s00394-015-0938-6. Epub 2015 May 29.
Recently, adrenomedullin (ADM) was defined as a new member of the adipokine family. ADM secreted by adipocytes, through its vasodilator and antioxidant actions, might be protective against metabolic syndrome-associated cardiovascular complications. The aim of the study was to assess plasma mid-regional (MR)-proADM levels in obese adolescents compared to normal-weight subjects and its relation with BMI, body composition and metabolic indices.
Plasma MR-proADM was measured in 32 healthy adolescents [BMI z-score (mean ± SEM) = 0.6 ± 0.09 and 0.8 ± 0.07 in females and males, respectively] and in 51 age-matched obese adolescents [BMI z-score (mean ± SEM) = 2.8 ± 0.12 and 2.9 ± 0.08 in female and males, respectively] by a time-resolved amplified cryptate emission technology assay.
Plasma MR-proADM levels resulted significantly higher in obese than in normal-weight adolescents (MR-proADM: 0.33 ± 0.1 vs 0.40 ± 0.1 nmol/L, p < 0.0001). Using univariate analysis, we observed that MR-proADM correlated significantly with BMI z-score (p < 0.0001), fat mass (p < 0.0001), circulating insulin (p < 0.004), HOMA-IR (p < 0.005), total cholesterol (p < 0.03) and LDL-cholesterol (p < 0.05). Including MR-proADM as response variable and its significant correlates into a multiple regression analysis, we observed that fat mass (p = 0.014) and BMI z-score (p = 0.036) were independent determinants of circulating MR-proADM.
Our study shows for the first time that obese adolescents have higher circulating levels of MR-proADM compared with normal-weight, appropriate controls suggesting its important involvement in obese patients.
最近，肾上腺髓质素（ADM）被定义为脂肪因子家族的新成员。脂肪细胞分泌的ADM通过其血管舒张和抗氧化作用，可能对代谢综合征相关的心血管并发症具有保护作用。本研究的目的是评估肥胖青少年与正常体重受试者相比血浆中段（MR）-proADM水平，及其与BMI、身体成分和代谢指标的关系。
采用时间分辨荧光增强隐色螯合物发射技术检测32名健康青少年[女性和男性的BMI z评分（均值±标准误）分别为0.6±0.09和0.8±0.07]和51名年龄匹配的肥胖青少年[女性和男性的BMI z评分（均值±标准误）分别为2.8±0.12和2.9±0.08]的血浆MR-proADM水平。
肥胖青少年的血浆MR-proADM水平显著高于正常体重青少年（MR-proADM：0.33±0.1对0.40±0.1 nmol/L，p<0.0001）。单因素分析显示，MR-proADM与BMI z评分（p<0.0001）、脂肪量（p<0.0001）、循环胰岛素（p<0.004）、HOMA-IR（p<0.005）、总胆固醇（p<0.03）和低密度脂蛋白胆固醇（p<0.05）显著相关。将MR-proADM作为反应变量及其显著相关因素纳入多元回归分析，发现脂肪量（p=0.014）和BMI z评分（p=0.036）是循环MR-proADM的独立决定因素。
我们的研究首次表明，与正常体重的合适对照相比，肥胖青少年的循环MR-proADM水平更高，提示其在肥胖患者中具有重要作用。
