Ma Shouguang, Zhang Demin, Zhang Wenjun, Wang Yinong
Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University , Ningbo , P. R. China.
Biotechnol Biotechnol Equip. 2014 Jan 2;28(1):27-32. doi: 10.1080/13102818.2014.901679.
The aim of this study was to obtain a nitrite-oxidizing bacterium with high nitrite oxidation activity for controlling nitrite levels. A nitrite-oxidizing bacterium, ZS-1, was isolated from the water of a coastal -rearing pond. The strain was identified as based on the phylogenetic analyses of the 16S ribosomal ribonucleic acid gene and sequence of ZS-1. Under aerobic condition, the nitrite-oxidizing activity of ZS-1 did not change considerably in the range of pH 7-9, but was strongly inhibited by lower (pH = 6) and higher (pH = 10) pH values. The optimum temperature range is 25-32 °C. Lower temperature made the adaptive phase of ZS-1 longer but did not affect its maximum nitrite oxidization rate. The nitrite-oxidizing activity of ZS-1 started to be inhibited by ammonia and nitrate when the concentrations of ammonia and nitrate reached 25 mg L and 100 mg L, respectively. The inhibition was stronger with higher concentration of ammonia or nitrate. The nitrite-oxidizing activity of ZS-1, however, was not inhibited by high concentration of nitrite (500 mg L). The nitrite-oxidizing activity of ZS-1 was increased by low ammonia concentration (1 mg L to 10 mg L).
本研究的目的是获得一株具有高亚硝酸盐氧化活性的亚硝酸盐氧化细菌，用于控制亚硝酸盐水平。从沿海养殖池塘的水中分离出一株亚硝酸盐氧化细菌ZS-1。基于ZS-1的16S核糖体核糖核酸基因和序列的系统发育分析，该菌株被鉴定为 。在有氧条件下，ZS-1的亚硝酸盐氧化活性在pH 7-9范围内变化不大，但在较低（pH = 6）和较高（pH = 10）的pH值下受到强烈抑制。最适温度范围是25-32℃。较低的温度使ZS-1的适应期延长，但不影响其最大亚硝酸盐氧化速率。当氨和硝酸盐的浓度分别达到25 mg/L和100 mg/L时，ZS-1的亚硝酸盐氧化活性开始受到氨和硝酸盐的抑制。氨或硝酸盐浓度越高，抑制作用越强。然而，ZS-1的亚硝酸盐氧化活性不受高浓度亚硝酸盐（500 mg/L）的抑制。低氨浓度（1 mg/L至10 mg/L）可提高ZS-1的亚硝酸盐氧化活性。
