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SPM1605对柯萨奇病毒B3的抗病毒活性。

Antiviral activity of SPM1605 against Coxsackievirus B3.

作者信息

Kim Min Ji, Lee Do Kyung, Park Jae Eun, Park Il Ho, Seo Jae Gu, Ha Nam Joo

机构信息

College of Pharmacy, Sahmyook University , Seoul , Republic of Korea.

R&D Center, Cellbiotech, Co. Ltd. , Gimpo , Republic of Korea.

出版信息

Biotechnol Biotechnol Equip. 2014 Jul 4;28(4):681-688. doi: 10.1080/13102818.2014.945237. Epub 2014 Oct 20.

DOI:10.1080/13102818.2014.945237
PMID:26019554
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4433936/
Abstract

Bifidobacteria are considered one of the most beneficial probiotics and have been widely studied for their effects against specific pathogens. The present study investigated the antiviral activity of probiotics isolated from Koreans against Coxsackievirus B3 (CVB3). The effect of probiotic isolates against CVB3 was measured by the plaque assay and cellular toxicity of bifidobacteria in HeLa cells was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Among 13 probiotic isolates, 3 , 2 and 1 had an antiviral effect against CVB3, while the others did not show such effect. SPM1605 showed the greatest inhibitory properties against CVB3. When the threshold cycle (CT) values for the treated SPM1605 samples were compared to the results for the non-treated samples, it was shown that the amplified viral sequences from the CVB3 had their copy number lowered by SPM1605. Moreover, the gene expression in infected HeLa cells was also inhibited by 50%. The results suggest that SPM1605 suppresses CVB3 and could be used as an alternative therapy against infectious diseases caused by coxsackieviruses.

摘要

双歧杆菌被认为是最有益的益生菌之一，并且已经针对其对特定病原体的作用进行了广泛研究。本研究调查了从韩国人分离出的益生菌对柯萨奇病毒B3（CVB3）的抗病毒活性。通过噬斑测定法测量益生菌分离株对CVB3的作用，并使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）测定法测量双歧杆菌在HeLa细胞中的细胞毒性。在13株益生菌分离株中，3株、2株和1株对CVB3有抗病毒作用，而其他菌株未显示出这种作用。SPM1605对CVB3表现出最大的抑制特性。当将处理过的SPM1605样品的阈值循环（CT）值与未处理样品的结果进行比较时，结果表明，来自CVB3的扩增病毒序列的拷贝数被SPM1605降低。此外，感染的HeLa细胞中的基因表达也被抑制了50%。结果表明，SPM1605可抑制CVB3，并可作为治疗由柯萨奇病毒引起的传染病的替代疗法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0b6/4433936/39c0d726f061/tbeq-28-681-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0b6/4433936/a4cb93c4c9f6/tbeq-28-681-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0b6/4433936/00b8cfa08a09/tbeq-28-681-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0b6/4433936/179ff28092dd/tbeq-28-681-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0b6/4433936/39c0d726f061/tbeq-28-681-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0b6/4433936/a4cb93c4c9f6/tbeq-28-681-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0b6/4433936/00b8cfa08a09/tbeq-28-681-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0b6/4433936/179ff28092dd/tbeq-28-681-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0b6/4433936/39c0d726f061/tbeq-28-681-g004.jpg

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