Thompson Katherine G, Rainer Barbara M, Antonescu Corina, Florea Liliana, Mongodin Emmanuel F, Kang Sewon, Chien Anna L
Department of Dermatology, Johns Hopkins University, Baltimore, MD, USA.
Department of Dermatology, Medical University of Graz, Graz, Austria.
Ann Dermatol. 2020 Feb;32(1):21-30. doi: 10.5021/ad.2020.32.1.21. Epub 2020 Jan 9.
Associations between acne and gastrointestinal comorbidities suggest that microbial dysbiosis and intestinal permeability may promote inflammatory acne, a condition often managed with oral antibiotics.
We performed a case-control study to investigate the skin and gut microbiota in 8 acne patients before and after receiving oral minocycline compared to controls matched by age ±5 years, sex, and race.
DNA was extracted from stool samples and facial skin swabs. Sequencing of the V3V4 region of the bacterial 16S rRNA gene was performed using Illumina MiSeq and analyzed using QIIME/MetaStats 2.0 software.
Acne patients included 7 female and 1 male, ages 20~32. Shannon diversity was not significantly different between the skin (=0.153) or gut (<0.999) microbiota of acne patients before and after antibiotics. The gut microbiota in pre-antibiotic acne patients compared to acne-free controls was depleted in probiotics (=0.001), (=0.001), and (=0.026). After antibiotics, the gut microbiota of acne patients was depleted in (=0.001), (=0.002), (=0.010), and (=0.042), while the skin microbiota was enriched in probiotics (=0.028) and (=0.029) and depleted in (=0.009) and (=0.028). At the phylum level, significant enrichment of Bacteroidetes in stool of acne patients following antibiotic treatment (=0.033) led to a decreased Firmicutes to Bacteroidetes ratio.
Minocycline produces significant derangements in the microbiota of the skin and gut, including many probiotic species, highlighting the potential for more targeted antimicrobial treatments for acne.
痤疮与胃肠道合并症之间的关联表明，微生物群落失调和肠道通透性可能会促进炎性痤疮，这种病症通常采用口服抗生素进行治疗。
我们开展了一项病例对照研究，以调查8名痤疮患者在接受口服米诺环素前后的皮肤和肠道微生物群，并与年龄相差±5岁、性别和种族相匹配的对照组进行比较。
从粪便样本和面部皮肤拭子中提取DNA。使用Illumina MiSeq对细菌16S rRNA基因的V3V4区域进行测序，并使用QIIME/MetaStats 2.0软件进行分析。
痤疮患者包括7名女性和1名男性，年龄在20至32岁之间。抗生素治疗前后，痤疮患者皮肤（=0.153）或肠道（<0.999）微生物群的香农多样性无显著差异。与无痤疮对照组相比，抗生素治疗前痤疮患者肠道微生物群中的益生菌（=0.001）、（=0.001）和（=0.026）减少。抗生素治疗后，痤疮患者肠道微生物群中的（=0.001）、（=0.002）、（=0.010）和（=0.042）减少，而皮肤微生物群中的益生菌（=0.028）和（=0.029）增加，（=0.009）和（=0.028）减少。在门水平上，抗生素治疗后痤疮患者粪便中拟杆菌门显著富集（=0.033），导致厚壁菌门与拟杆菌门的比例降低。
米诺环素会导致皮肤和肠道微生物群发生显著紊乱，包括许多益生菌种类，这突出了对痤疮进行更具针对性抗菌治疗的潜力。
