Ge Hong-Lian, Zhang Fu-Li
College of Life Science and Agronomy, Zhoukou Normal University , Zhoukou 466001 , China.
Biotechnol Biotechnol Equip. 2015 Mar 4;29(2):289-298. doi: 10.1080/13102818.2014.999298. Epub 2015 Jan 13.
The aim of this study was to investigate whether the composite photosynthetic bacterial inoculant PS21 alleviate the damage inflicted on wheat seedlings by tetrabromobisphenol A (TBBPA). The biochemical characteristics of wheat seedlings were analysed through laboratory simulation after co-treating seedlings with PS21 and 0, 5, 10, 20, 40, 80 and 100 mg kg TBBPA, respectively. The results showed that TBBPA reduced the total chlorophyll content and increased the malondialdehyde (MDA) content. TBBPA increased the soluble sugar content, soluble protein content and activate superoxide dismutase (SOD; EC: 1.15.1.1), catalase (CAT; EC: 1.11.1.6) and peroxidase (POD; EC: 1.11.1.7) at low concentrations, while it reduced soluble sugar content, soluble protein content and decreased the activities of SOD, CAT and POD at high concentrations. At the concentration of 10 CFU mL, PS21 could markedly relieve the toxicity of different concentrations of TBBPA on wheat seedlings. Wheat seedlings treated with both TBBPA and PS21 showed a higher soluble sugar content, higher soluble protein content, higher SOD, CAT and POD activities, and a lower MDA content as compared to those treated only with TBBPA. The composite photosynthetic bacterial inoculant PS21 significantly alleviates the damage inflicted on wheat seedlings by TBBPA.
本研究旨在探究复合光合细菌菌剂PS21是否能减轻四溴双酚A（TBBPA）对小麦幼苗造成的损害。分别用PS21与0、5、10、20、40、80和100 mg/kg的TBBPA共同处理小麦幼苗后，通过实验室模拟分析小麦幼苗的生化特性。结果表明，TBBPA降低了总叶绿素含量，增加了丙二醛（MDA）含量。低浓度时，TBBPA增加了可溶性糖含量、可溶性蛋白含量，并激活了超氧化物歧化酶（SOD；酶编号：1.15.1.1）、过氧化氢酶（CAT；酶编号：1.11.1.6）和过氧化物酶（POD；酶编号：1.11.1.7）的活性，而高浓度时，它降低了可溶性糖含量、可溶性蛋白含量，并降低了SOD、CAT和POD的活性。在浓度为10 CFU/mL时，PS21能显著减轻不同浓度TBBPA对小麦幼苗的毒性。与仅用TBBPA处理的小麦幼苗相比，用TBBPA和PS21共同处理的小麦幼苗表现出更高的可溶性糖含量、更高的可溶性蛋白含量、更高的SOD、CAT和POD活性以及更低的MDA含量。复合光合细菌菌剂PS21能显著减轻TBBPA对小麦幼苗造成的损害。
