含胞嘧啶 - 胸腺嘧啶类似物的寡核苷酸的合成与双链稳定性
Synthesis and duplex stability of oligonucleotides containing cytosine-thymine analogues.
作者信息
Lin P K, Brown D M
机构信息
MRC Laboratory of Molecular Biology, Cambridge, UK.
出版信息
Nucleic Acids Res. 1989 Dec 25;17(24):10373-83. doi: 10.1093/nar/17.24.10373.
Abstract
The synthesis of the deoxynucleoside derived from the base P, 6H,8H-3,4-dihydro-pyrimido[4,5-c] [4,5-c] [1,2]oxazin-7-one, 2, and its introduction by established phosphoramidite and H-phosphonate chemistry into oligonucleotides is described. The melting transition temperatures (Tm) of a range of heptadecamer duplexes containing P/A and P/G base-pairs are compared with corresponding ones having N4-methoxycytosine (M) 1 and mismatched normal bases. P/A and P/G pairs allow closely similar duplex stabilities and have the potential to reduce the multiplicity of probes and primers based on amino acid sequences by removing the T/C degeneracy.
摘要
描述了由碱基P（6H，8H - 3,4 - 二氢 - 嘧啶并[4,5 - c][4,5 - c][1,2]恶嗪 - 7 - 酮）衍生的脱氧核苷的合成，以及通过既定的亚磷酰胺和H - 膦酸酯化学方法将其引入寡核苷酸的过程。将一系列含有P/A和P/G碱基对的十七聚体双链体的熔解转变温度（Tm）与具有N4 - 甲氧基胞嘧啶（M）1和错配的正常碱基的相应双链体进行了比较。P/A和P/G碱基对具有非常相似的双链体稳定性，并且有可能通过消除T/C简并性来减少基于氨基酸序列的探针和引物的多样性。
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