Department of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Ny Munkegade 118, 8000, Aarhus C, Denmark.
Biomaterials. 2015 Sep;62:58-65. doi: 10.1016/j.biomaterials.2015.05.026. Epub 2015 May 16.
Topographical features on the nanometer scale are known to influence cellular behavior. The response of specific cell types to various types of surface structures is currently still being investigated. Alumina ceramics play an important role as biomaterials, e.g., in medical and dental applications. In this study, we investigated the influence of nanoscale surface features with low aspect ratio (< 0.1) on the response of osteoblast-like MG-63 cells. To this end, low-energy ion irradiation was employed to produce shallow nanoscale ripple patterns on Al2O3(0001) surfaces with lateral periodicities of 24 nm and 179 nm and heights of only 0.7 and 11.5 nm, respectively. The nanopatterning was found to increase the proliferation of MG-63 cells and may lead to pseudopodia alignment along the ripples. Furthermore, focal adhesion behavior and cell morphology were analyzed. We found that MG-63 cells are able to recognize surface nanopatterns with extremely low vertical variations of less than 1 nm. In conclusion, it is shown that surface topography in the sub-nm range significantly influences the response of osteoblast-like cells.
纳米尺度的形貌特征被认为会影响细胞行为。目前，人们仍在研究特定细胞类型对各种表面结构的反应。氧化铝陶瓷作为生物材料具有重要作用，例如在医疗和牙科应用中。在这项研究中，我们研究了低纵横比（<0.1）的纳米级表面特征对成骨样 MG-63 细胞反应的影响。为此，采用低能离子辐照在 Al2O3(0001)表面上产生具有 24nm 和 179nm 横向周期性以及仅 0.7nm 和 11.5nm 高度的浅纳米波纹图案。结果表明，纳米图案化可以增加 MG-63 细胞的增殖，并可能导致伪足沿着波纹排列。此外，还分析了细胞的黏附行为和形态。我们发现 MG-63 细胞能够识别具有小于 1nm 超低垂直变化的表面纳米图案。总之，结果表明亚纳米范围内的表面形貌会显著影响成骨样细胞的反应。
