Ghavipanjeh Gholam Reza, Pourshanazari Ali Asghar, Alaei Hojjatllah, Karimi Sara, Nejad Meghdad Abarghouei
Department of Physiology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran, postal code: 81746-73461.
Department of Physiology, Faculty of Sciences, Payamnoor Isfahan University, Isfahan, Iran, postal code: 81395-671.
Malays J Med Sci. 2015 Mar-Apr;22(2):33-40.
The dorsal raphe nucleus (DRN) influences a wide range of behavioral and reward function. In this study, we evaluated electrical stimulation and inactivation of DRN on morphine conditioned place preference (CPP).
The rats were anesthetised (n = 7 for each group) and the electrode and cannula were implanted into the DRN by stereotaxic instrument. Electrical stimulation (100μA) and reversible inactivation by lidocaine were induced into DRN and then morphine-induced CPP was investigated.
The stimulation of DRN in combination with effective dose of morphine showed a significant decrease only on expression phases 20s (SD 33.7) when compared with morphine group 119.85s (SD 23.7) (One way ANOVA, Tukey's; P = 0.036). Also, this stimulation in combination with ineffective dose of morphine showed a significant increase only on acquisition phases 67.5s (SD 41.2) of CPP compared with morphine group -46s (SD 18.51) (P = 0.034). Also, there were not significant differences in inactivation of DRN by lidocaine on different phase of CPP (P = 0.091) CONCLUSION: It is possible that electrical stimulation of the DRN with changes in concentration of serotonin or involving other transmitters such as glutamate and gamma amino butyric acid (GABA) would be involved to these changes of CPP.
中缝背核（DRN）影响广泛的行为和奖赏功能。在本研究中，我们评估了中缝背核的电刺激和失活对吗啡条件性位置偏爱（CPP）的影响。
将大鼠麻醉（每组n = 7），通过立体定位仪将电极和套管植入中缝背核。对中缝背核进行电刺激（100μA）和利多卡因可逆性失活，然后研究吗啡诱导的CPP。
与吗啡组119.85秒（标准差23.7）相比，中缝背核刺激联合有效剂量吗啡仅在表达阶段20秒（标准差33.7）时显著降低（单因素方差分析，Tukey检验；P = 0.036）。此外，与吗啡组-46秒（标准差18.51）相比，该刺激联合无效剂量吗啡仅在CPP获得阶段67.5秒（标准差41.2）时显著增加（P = 0.034）。此外，利多卡因对中缝背核的失活在CPP不同阶段无显著差异（P = 0.091）。结论：中缝背核的电刺激可能通过血清素浓度变化或涉及其他递质如谷氨酸和γ-氨基丁酸（GABA）参与CPP的这些变化。
