用于生物大分子溶液纯化、生物物理及X射线分析的自动化流程
Automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions.
作者信息
Graewert Melissa A, Franke Daniel, Jeffries Cy M, Blanchet Clement E, Ruskule Darja, Kuhle Katja, Flieger Antje, Schäfer Bernd, Tartsch Bernd, Meijers Rob, Svergun Dmitri I
机构信息
European Molecular Biology Laboratory (EMBL) Hamburg, 22607 Hamburg, Germany.
Robert Koch-Institut, Division of Enteropathogenic Bacteria and Legionella (FG11), Burgstr. 37, 38855 Wernigerode, Germany.
出版信息
Sci Rep. 2015 Jun 1;5:10734. doi: 10.1038/srep10734.
Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg).
小角X射线散射（SAXS）是一种在溶液中对生物大分子进行结构分析的越来越流行的方法，但常常受到样品固有多分散性的阻碍。我们开发了一种一体化系统，该系统将在线样品组分分离与对分离组分的并行生物物理和SAXS表征相结合。与自动化数据分析管道相结合的该系统为在P12同步加速器光束线（PETRA-3，EMBL/德国电子同步加速器研究所，汉堡）研究难处理的样品提供了一种新工具。
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