通过定量 SAXS 分析绘制的综合大分子构象图。
Comprehensive macromolecular conformations mapped by quantitative SAXS analyses.
出版信息
Nat Methods. 2013 Jun;10(6):453-4. doi: 10.1038/nmeth.2453.
Abstract
Comprehensive perspectives of macromolecular conformations are required to connect structure to biology. Here we present a small angle X-ray scattering (SAXS) Structural Similarity Map (SSM) and Volatility of Ratio () metric providing comprehensive, quantitative and objective (superposition-independent) perspectives on solution state conformations. We validate and SSM utility on human MutSβ, a key ABC ATPase and chemotherapeutic target, by revealing MutSβ DNA sculpting and identifying multiple conformational states for biological activity.
摘要
需要综合的大分子构象视角将结构与生物学联系起来。在这里，我们提出了一种小角度 X 射线散射（SAXS）结构相似性图谱（SSM）和比率变异性（）度量，为溶液状态构象提供了全面、定量和客观（不依赖于叠加）的视角。我们通过揭示 MutSβ 的 DNA 造型并确定了多个生物活性构象状态，验证了和 SSM 在人类 MutSβ 中的实用性，MutSβ 是一种关键的 ABC ATP 酶和化学治疗靶点。
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