Pinto Clidia E M, Farias Davi F, Carvalho Ana F U, Oliveira José T A, Pereira Mirella L, Grangeiro Thalles B, Freire José E C, Viana Daniel A, Vasconcelos Ilka M
Department of Biochemistry and Molecular Biology, Federal University of Ceará, Campus do Pici, 60440-900, Fortaleza, CE, Brazil.
Department of Biochemistry and Molecular Biology, Federal University of Ceará, Campus do Pici, 60440-900, Fortaleza, CE, Brazil.
Food Chem Toxicol. 2015 Sep;83:1-9. doi: 10.1016/j.fct.2015.05.012. Epub 2015 May 30.
Mo-CBP3 is an antifungal protein produced by Moringa oleifera which has been investigated as potential candidate for developing transgenic crops. Before the use of novel proteins, food safety tests must be conducted. This work represents an early food safety assessment of Mo-CBP3, using the two-tiered approach proposed by ILSI. The history of safe use, mode of action and results for amino acid sequence homology using the full-length and short contiguous amino acids sequences indicate low risk associated to this protein. Mo-CBP3 isoforms presented a reasonable number of alignments (>35% identity) with allergens in a window of 80 amino acids. This protein was resistant to pepsin degradation up to 2 h, but it was susceptible to digestion using pancreatin. Many positive attributes were presented for Mo-CBP3. However, this protein showed high sequence homology with allergens and resistance to pepsin digestion that indicates that further hypothesis-based testing on its potential allergenicity must be done. Additionally, animal toxicity evaluations (e.g. acute and repeated dose oral exposure assays) must be performed to meet the mandatory requirements of several regulatory agencies. Finally, the approach adopted here exemplified the importance of performing an early risk assessment of candidate proteins for use in plant transformation programs.
辣木几丁质结合蛋白3（Mo-CBP3）是一种由辣木产生的抗真菌蛋白，已被作为开发转基因作物的潜在候选物进行研究。在使用新型蛋白质之前，必须进行食品安全测试。这项工作采用国际生命科学学会（ILSI）提出的两级方法，对Mo-CBP3进行了早期食品安全评估。其安全使用历史、作用方式以及使用全长和短连续氨基酸序列进行氨基酸序列同源性分析的结果表明，该蛋白风险较低。在80个氨基酸的窗口内，Mo-CBP3亚型与过敏原的比对数量合理（同一性>35%）。该蛋白在长达2小时的时间内对胃蛋白酶降解具有抗性，但对胰蛋白酶消化敏感。Mo-CBP3具有许多积极特性。然而，该蛋白与过敏原具有高度序列同源性且对胃蛋白酶消化具有抗性，这表明必须对其潜在致敏性进行进一步的基于假设的测试。此外，必须进行动物毒性评估（如急性和重复剂量口服暴露试验），以满足多个监管机构的强制要求。最后，这里采用的方法例证了对用于植物转化计划的候选蛋白进行早期风险评估的重要性。
