AgResearch, Hopkirk Research Institute, Palmerston North, New Zealand.
School of Chemical and Physical Sciences, Victoria University of Wellington, Wellington, New Zealand.
PLoS One. 2023 Jan 19;18(1):e0278853. doi: 10.1371/journal.pone.0278853. eCollection 2023.
Bronchopneumonia is a common respiratory disease in livestock. Mannheimia haemolytica is considered the main causative pathogen leading to lung damage in sheep, with Mycoplasma ovipneumoniae and ParaInfluenza virus type 3, combined with adverse physical and physiological stress, being predisposing factors. A balance of humoral and cellular immunity is thought to be important for protection against developing respiratory disease. In the current study, we compared the ability of the trehalose glycolipid adjuvant C18Brar (C18-alkylated brartemicin analogue) and three commercially available adjuvant systems i.e., Quil-A, Emulsigen-D, and a combination of Quil-A and aluminium hydroxide gel, to stimulate antibody and cellular immune responses to antigens from inactivated whole cells of M. haemolytica and M. ovipneumoniae in sheep. C18Brar and Emulsigen-D induced the strongest antigen-specific antibody responses to both M. haemolytica and M. ovipneumoniae, while C18Brar and Quil-A promoted the strongest antigen-specific IL-17A responses. The expression of genes with known immune functions was determined in antigen-stimulated blood cultures using Nanostring nCounter technology. The expression levels of CD40, IL22, TGFB1, and IL2RA were upregulated in antigen-stimulated blood cultures from animals vaccinated with C18Brar, which is consistent with T-cell activation. Collectively, the results demonstrate that C18Brar can promote both antibody and cellular responses, notably Th17 immune responses in a ruminant species.
绵羊支气管肺炎是一种常见的家畜呼吸道疾病。溶血曼海姆菌被认为是导致绵羊肺部损伤的主要病原体，而绵羊肺炎支原体和 3 型副流感病毒与不利的物理和生理应激一起，是诱发因素。体液和细胞免疫的平衡被认为对预防呼吸道疾病的发生很重要。在本研究中，我们比较了海藻糖糖脂佐剂 C18Brar（C18-烷基化 brartemicin 类似物）和三种市售佐剂系统（Quil-A、Emulsigen-D 和 Quil-A 与氢氧化铝凝胶的组合）刺激绵羊对灭活的完整曼海姆菌和绵羊肺炎支原体细胞抗原的抗体和细胞免疫反应的能力。C18Brar 和 Emulsigen-D 诱导针对曼海姆菌和绵羊肺炎支原体的最强的抗原特异性抗体反应，而 C18Brar 和 Quil-A 则促进最强的抗原特异性 IL-17A 反应。使用 Nanostring nCounter 技术，在抗原刺激的血液培养物中确定了具有已知免疫功能的基因的表达。用 C18Brar 免疫接种的动物的抗原刺激的血液培养物中，CD40、IL22、TGFB1 和 IL2RA 的表达上调，这与 T 细胞激活一致。总的来说，结果表明 C18Brar 可以促进抗体和细胞反应，特别是在反刍动物中促进 Th17 免疫反应。
