Nicoletti Gabrieli, Cipolatti Eliane P, Valério Alexsandra, Carbonera NatáliaThaisa Gamba, Soares Nicole Spillere, Theilacker Eron, Ninow Jorge L, de Oliveira Débora
Chemical and Food Engineering Department, Federal University of Santa Catarina (UFSC), PO Box 476, Florianópolis, SC, 88040-900, Brazil.
Bioprocess Biosyst Eng. 2015 Sep;38(9):1739-48. doi: 10.1007/s00449-015-1415-6. Epub 2015 Jun 3.
With the aim of studying the best method for the interaction of polyurethane (PU) foam and Candida antarctica lipase B, different methods of CalB immobilization were studied: adsorption (PU-ADS), bond (using polyethyleneimine) (PU-PEI), ionic adsorption by PEI with cross-linking with glutaraldehyde (PU-PEI-GA) and entrapment (PU). The characterization of immobilized enzyme derivatives was performed by apparent density and Fourier transform infrared spectroscopy. The free enzyme and enzyme preparations were evaluated at different pH values and temperatures. The highest enzyme activity was obtained using the PU method (5.52 U/g). The methods that stood out to compare the stabilities and kinetic parameters were the PU and PU-ADS. Conversions of 83.5 and 95.9 % for PU and PU-ADS derivatives were obtained, in 24 h reaction, using citronella oil and propionic acid as substrates.
为了研究聚氨酯（PU）泡沫与南极假丝酵母脂肪酶B相互作用的最佳方法，研究了不同的固定化南极假丝酵母脂肪酶B的方法：吸附法（PU-ADS）、键合法（使用聚乙烯亚胺）（PU-PEI）、聚乙烯亚胺离子吸附并与戊二醛交联法（PU-PEI-GA）和包埋法（PU）。通过表观密度和傅里叶变换红外光谱对固定化酶衍生物进行表征。在不同pH值和温度下对游离酶和酶制剂进行评估。采用PU法获得了最高的酶活性（5.52 U/g）。在比较稳定性和动力学参数方面表现突出的方法是PU法和PU-ADS法。以香茅油和丙酸为底物，在24小时反应中，PU和PU-ADS衍生物的转化率分别为83.5%和95.9%。
