Heredia Antonio, Heredia-Guerrero José Alejandro, Domínguez Eva
a IHSM UMA-CSIC; Departamento de Biología Molecular y Bioquímica; Universidad de Málaga ; Málaga , Spain.
Plant Signal Behav. 2015;10(5):e1019979. doi: 10.1080/15592324.2015.1019979.
Tomato fruits (Solanum lycopersicum L.) accumulate flavonoids in their cuticle and epidermal cells during ripening. These flavonoids come from de novo biosynthesis due to a significant increase in chalcone synthase (CHS) activity during ripening. Virus-induced gene silencing (VIGS) of tomato fruits have been used to down-regulate SlCHS expression during ripening and analyze the effects at the epidermal and cuticle level. Besides the expected change in fruit color due to a lack of flavonoids incorporated to the cuticle, several other modifications such as a decrease in the amount of cutin and polysaccharides were observed. These indicate a role for either flavonoids or CHS in the alteration of the expression levels of some genes involved in cuticle biosynthesis. Moreover, a negative interaction between the 2 cuticle components, flavonoids and waxes, suggests a relationship between these 2 metabolic pathways.
番茄果实（番茄）在成熟过程中，其角质层和表皮细胞会积累黄酮类化合物。这些黄酮类化合物源于从头生物合成，因为在成熟过程中查尔酮合酶（CHS）活性显著增加。番茄果实的病毒诱导基因沉默（VIGS）已被用于在成熟过程中下调SlCHS表达，并在表皮和角质层水平分析其影响。除了由于角质层中缺乏黄酮类化合物而导致果实颜色出现预期变化外，还观察到了其他一些变化，如角质和多糖含量减少。这些表明黄酮类化合物或CHS在改变一些参与角质层生物合成的基因表达水平中发挥作用。此外，角质层的两个成分黄酮类化合物和蜡之间存在负相互作用，这表明这两个代谢途径之间存在关联。
