Behbod Fariba, Vivanco Maria D M
Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA,
Methods Mol Biol. 2015;1293:73-81. doi: 10.1007/978-1-4939-2519-3_4.
The side population (SP) assay has been utilized as a method for isolation and characterization of normal and cancer stem cells from a variety of tissues. However, the SP phenotype may not be a common property of all stem cells. This chapter reviews the principle and potential pitfalls of the SP assay with an emphasis on mammary gland SP cell analysis.
侧群(SP)分析已被用作从多种组织中分离和鉴定正常及癌症干细胞的一种方法。然而,SP表型可能并非所有干细胞的共同特性。本章回顾了SP分析的原理和潜在缺陷,重点是乳腺SP细胞分析。