NorLux Neuro-Oncology Laboratory, Oncology Department, Centre de Recherche Public de la Santé, 84 Val Fleuri, Luxembourg.
Cell Stem Cell. 2011 Feb 4;8(2):136-47. doi: 10.1016/j.stem.2011.01.007.
The "Side Population" (SP) discrimination assay is a flow cytometry method used to detect stem cells based on the dye efflux properties of ABC transporters. We discuss the SP assay and its applications in stem cell biology, with an emphasis on the technical challenges related to sample preparation, data acquisition, analysis, and interpretation. We highlight the value of multicolor phenotyping, the impact of DNA ploidy, and the importance of distinguishing graft versus host cells for an appropriate SP discrimination. To improve the consistency and reliability of data between laboratories, we propose a set of recommendations for SP assay data reporting.
“侧群”（SP）区分检测是一种基于 ABC 转运蛋白的染料外排特性来检测干细胞的流式细胞术方法。我们讨论了 SP 检测及其在干细胞生物学中的应用，重点介绍了与样品制备、数据采集、分析和解释相关的技术挑战。我们强调了多色表型分析的价值、DNA 倍性的影响以及区分移植物与宿主细胞对于适当的 SP 区分的重要性。为了提高实验室间数据的一致性和可靠性，我们提出了一组关于 SP 检测数据报告的建议。
