Hasegawa Yuri, Miura Kiyonori, Higashijima Ai, Abe Shuhei, Miura Shoko, Yoshiura Koh-ichiro, Masuzaki Hideaki
Department of Obstetrics and Gynecology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Department of Obstetrics and Gynecology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Reprod Sci. 2015 Dec;22(12):1569-76. doi: 10.1177/1933719115589407. Epub 2015 Jun 2.
The aim of this study was to clarify the association between placenta previa and circulating levels of cell-free pregnancy-associated placenta-specific microRNAs (miRNAs) in maternal plasma.
Twenty singleton pregnancies with placenta previa (placenta previa group) and 26 uncomplicated pregnancies (control group) were recruited. Blood sampling was performed at 32 weeks of gestation, and cesarean delivery in all cases of placenta previa was performed at a mean gestational age of 37 weeks. The maternal plasma concentrations of cell-free pregnancy-associated placenta-specific miRNAs (miR-517a and miR-518b) were measured by absolute quantitative real-time reverse transcription-polymerase chain reaction.
Plasma concentrations of cell-free miR-517a were significantly higher in the placenta previa group than that in the control group (P = .011), while the plasma concentration of cell-free miR-518b was significantly lower in the placenta previa group than that in the control group (P = .004). Plasma concentrations of cell-free miR-517a in placenta previa were significantly higher in placenta previa with alert bleeding later group than those in placenta previa without alert bleeding group or control group (P = .030 or .047, respectively) and correlated with the volume of hemorrhage at delivery (R and P value: .512 and .025).
Plasma concentrations of cell-free miR-517a and miR-518b at 32 weeks of gestation were altered in pregnant women with placenta previa, and the circulating level of cell-free miR-517a in placenta previa may be a predictive marker for the risks of alert bleeding later and massive hemorrhage at delivery.
本研究旨在阐明前置胎盘与孕妇血浆中无细胞妊娠相关胎盘特异性微小RNA（miRNA）循环水平之间的关联。
招募了20例单胎前置胎盘妊娠（前置胎盘组）和26例无并发症妊娠（对照组）。在妊娠32周时进行采血，所有前置胎盘病例均在平均孕周37周时进行剖宫产。通过绝对定量实时逆转录-聚合酶链反应测量孕妇血浆中无细胞妊娠相关胎盘特异性miRNA（miR-517a和miR-518b）的浓度。
前置胎盘组无细胞miR-517a的血浆浓度显著高于对照组（P = .011），而前置胎盘组无细胞miR-518b的血浆浓度显著低于对照组（P = .004）。前置胎盘伴晚期警戒性出血组无细胞miR-517a的血浆浓度显著高于前置胎盘无警戒性出血组或对照组（分别为P = .030或.047），且与分娩时出血量相关（R和P值：.512和.025）。
前置胎盘孕妇在妊娠32周时无细胞miR-517a和miR-518b的血浆浓度发生改变，前置胎盘时无细胞miR-517a的循环水平可能是晚期警戒性出血和分娩时大出血风险的预测标志物。
