Choi In Seong, Cho Eun Jin, Moon Jae-Hak, Bae Hyeun-Jong
Bio-energy Research Center, Chonnam National University, Gwangju 500-757, Republic of Korea.
Department of Food Science and Technology and Functional Food Research Center, Chonnam National University, Gwangju 500-757, Republic of Korea.
Food Chem. 2015 Dec 1;188:537-42. doi: 10.1016/j.foodchem.2015.05.028. Epub 2015 May 7.
Onion skin waste (OSW), which is produced from processed onions, is a major industrial waste. We evaluated the use of OSW for biosugar and quercetin production. The carbohydrate content of OSW was analyzed, and the optimal conversion conditions were evaluated by varying enzyme mixtures and loading volumes for biosugar production and quercetin extraction. The enzymatic conversion rate of OSW to biosugar was 98.5% at 0.72 mg of cellulase, 0.16 mg of pectinase, and 1.0mg of xylanase per gram of dry OSW. Quercetin extraction also increased by 1.61-fold after complete enzymatic hydrolysis. In addition, the newly developed nano-matrix (terpyridine-immobilized silica-coated magnetic nanoparticles-zinc (TSMNP-Zn matrix) was utilized to separate quercetin from OSW extracts. The nano-matrix facilitated easy separation and purification of quercetin. Using the TSMNP-Zn matrix the quercetin was approximately 90% absorbed. In addition, the recovery yield of quercetin was approximately 75% after treatment with ethylenediaminetetraacetic acid.
洋葱皮废料（OSW）是加工洋葱产生的主要工业废料。我们评估了利用OSW生产生物糖和槲皮素的可行性。分析了OSW的碳水化合物含量，并通过改变酶混合物以及生物糖生产和槲皮素提取的加样量来评估最佳转化条件。每克干燥的OSW在0.72毫克纤维素酶、0.16毫克果胶酶和1.0毫克木聚糖酶的作用下，OSW转化为生物糖的酶促转化率为98.5%。完全酶水解后，槲皮素的提取量也增加了1.61倍。此外，新开发的纳米基质（三联吡啶固定化二氧化硅包覆磁性纳米颗粒-锌（TSMNP-Zn基质））被用于从OSW提取物中分离槲皮素。该纳米基质便于槲皮素的分离和纯化。使用TSMNP-Zn基质时，槲皮素的吸附率约为90%。此外，用乙二胺四乙酸处理后，槲皮素的回收率约为75%。
