甲型流感病毒PA-X蛋白中对其关闭活性至关重要的区域的定位
Mapping of a Region of the PA-X Protein of Influenza A Virus That Is Important for Its Shutoff Activity.
作者信息
Oishi Kohei, Yamayoshi Seiya, Kawaoka Yoshihiro
机构信息
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
出版信息
J Virol. 2015 Aug;89(16):8661-5. doi: 10.1128/JVI.01132-15. Epub 2015 Jun 3.
Abstract
Influenza A virus PA-X comprises an N-terminal PA endonuclease domain and a C-terminal PA-X-specific domain. PA-X reduces host and viral mRNA accumulation via its endonuclease function. Here, we found that the N-terminal 15 amino acids, particularly six basic amino acids, in the C-terminal PA-X-specific region are important for PA-X shutoff activity. These six basic amino acids enabled a PA deletion mutant to suppress protein expression at a level comparable to that of wild-type PA-X.
摘要
甲型流感病毒PA-X由一个N端PA核酸内切酶结构域和一个C端PA-X特异性结构域组成。PA-X通过其核酸内切酶功能降低宿主和病毒mRNA的积累。在此，我们发现C端PA-X特异性区域的N端15个氨基酸，特别是六个碱性氨基酸，对PA-X的关闭活性很重要。这六个碱性氨基酸使PA缺失突变体能够在与野生型PA-X相当的水平上抑制蛋白质表达。
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