Ostash Bohdan, Yushchuk Oleksandr, Tistechok Stepan, Mutenko Halyna, Horbal Lilia, Muryn Andriy, Dacyuk Yuriy, Kalinowski Jorn, Luzhetskyy Andriy, Fedorenko Victor
Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, 4 Hrushevskoho st., Rm. 104, Lviv, 79005, Ukraine.
World J Microbiol Biotechnol. 2015 Aug;31(8):1297-301. doi: 10.1007/s11274-015-1882-6. Epub 2015 Jun 4.
Analysis of the draft sequence of the genome of teicoplanin producer Actinoplanes teichomyceticus (NRRL-B16726) led to identification of several genes encoding AraC-family regulators that resemble AdpA, master regulator of transcription in Streptomyces. We elucidated possible regulatory functions of one of the identified genes, adpA19(at), most similar to archetypal adpA from model Streptomyces species, in a series of expression experiments. Introduction of adpA19 at under control of its own promoter on moderate copy number vector pKC1139 into NRRL-B16726 had no influence on antibiotic production and sporulation. Introduction of adpA19 at into Streptomyces coelicolor M145 and several S. ghanaensis strains had major influence on antibiotic production by these bacteria. Finally, adpA19 at expression in a set of soil actinomycete isolates led to induction of synthesis of antibiotic compounds. Our data point to pleiotropic regulatory role of adpA19(at), warranting its use as a tool to manipulate secondary metabolome of actinomycetes.
对替考拉宁产生菌游动放线菌（NRRL-B16726）基因组草图序列的分析，导致鉴定出几个编码与链霉菌转录主调节因子AdpA相似的AraC家族调节因子的基因。我们在一系列表达实验中阐明了所鉴定的基因之一adpA19（at）的可能调节功能，该基因与模式链霉菌物种的原型adpA最相似。将在中等拷贝数载体pKC1139上由其自身启动子控制的adpA19 at导入NRRL-B16726中，对抗生素生产和孢子形成没有影响。将adpA19 at导入天蓝色链霉菌M145和几种加纳链霉菌菌株中，对这些细菌的抗生素生产有重大影响。最后，在一组土壤放线菌分离物中表达adpA19 at导致抗生素化合物合成的诱导。我们的数据表明adpA19（at）具有多效调节作用，这使其有理由用作操纵放线菌次级代谢组的工具。
