Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, 4 Hrushevskoho st. 4, Rm. 104, 79005 Lviv, Ukraine.
World J Microbiol Biotechnol. 2012 May;28(5):2095-100. doi: 10.1007/s11274-012-1013-6. Epub 2012 Feb 10.
Actinoplanes teichomyceticus produces the lipoglycopeptide antibiotic teicoplanin, which is considered a last line of defense against multidrug resistant Gram-positive cocci. Different strategies have been employed to generate industrial producers of teicoplanin, however they do not include manipulations of teicoplanin biosynthetic genes due to a poorly developed genetic "toolkit" for this strain. Through this work, we extend the choice of vectors that can be conjugally transferred and maintained in A. teichomyceticus. Antibiotic producing properties and stability of the transconjugants have been examined. As an illustration of the utility of pSG5-based vector pKC1139, we improved teicoplanin production by the wild type strain via manipulations of two regulatory genes from the teicoplanin biosynthetic cluster, tcp28 and tcp29.
游动放线菌产生糖肽类抗生素替考拉宁，被认为是对抗多种耐药革兰氏阳性球菌的最后一道防线。人们采用了不同的策略来生产替考拉宁的工业生产菌，但由于该菌株遗传“工具包”不发达，因此并未对替考拉宁生物合成基因进行操作。通过这项工作，我们扩展了可在游动放线菌中通过接合转移和维持的载体选择。已经检查了转导子的抗生素产生特性和稳定性。作为基于 pSG5 的载体 pKC1139 的实用性的说明，我们通过操纵替考拉宁生物合成簇中的两个调节基因 tcp28 和 tcp29 来提高野生型菌株的替考拉宁产量。
