Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, PR China.
Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.
Water Res. 2015 Sep 15;81:72-83. doi: 10.1016/j.watres.2015.05.012. Epub 2015 May 12.
The challenge of energy generation from biodegradation of recalcitrant organics in microbial fuel cells (MFCs) is mainly attributed to their persistence to degradation under anaerobic condition in anode chamber of MFCs. In this work, we demonstrated that electricity generation from aniline, a typical recalcitrant organic matter under anaerobic condition was remarkably facilitated by employing oxygen into bioanode of MFCs. By exposing bioanode to air, electrons of 47.2 ± 6.9 C were recovered with aniline removal efficiency of 91.2 ± 2.2% in 144 h. Limited oxygen supply (the anodic headspace was initially filled with air and then closed) resulted in the decrease of electrons recovery and aniline removal efficiency by 52.5 ± 9.4% and 74.2 ± 2.1%, respectively, and further decline by respective 64.3 ± 4.5% and 82.7 ± 1.0% occurred under anaerobic condition. Community analysis showed that anode biofilm was predominated by several aerobic aniline degrading bacteria (AADB) and anode-respiration bacteria (ARB), which likely cooperated with each other and finally featured the energy recovery from aniline. Cyclic voltammetry indicated that anodic bacteria transferred electrons to anode mainly through electron shuttle. This study provided a new sight to acquaint us with the positive role of oxygen in biodegradation of recalcitrant organics on anode as well as electricity generation.
在微生物燃料电池（MFC）中，从难生物降解有机物的生物降解中产生能量的挑战主要归因于它们在 MFC 的阳极室内的厌氧条件下持续存在降解的问题。在这项工作中，我们证明了通过向 MFC 的生物阳极中引入氧气，可以显著促进在厌氧条件下典型难生物降解有机物苯胺的发电。通过将生物阳极暴露于空气中，在 144 小时内，以 47.2 ± 6.9 C 的电子回收率和 91.2 ± 2.2%的苯胺去除效率去除了 47.2 ± 6.9 C 的电子。有限的氧气供应（阳极腔室最初充满空气，然后关闭）导致电子回收率和苯胺去除效率分别下降了 52.5 ± 9.4%和 74.2 ± 2.1%，在厌氧条件下进一步下降了 64.3 ± 4.5%和 82.7 ± 1.0%。群落分析表明，阳极生物膜主要由几种好氧苯胺降解菌（AADB）和阳极呼吸菌（ARB）组成，它们可能相互合作，最终实现了从苯胺中回收能量。循环伏安法表明，阳极细菌主要通过电子穿梭体将电子传递到阳极。这项研究为我们了解氧气在阳极难生物降解有机物生物降解和发电中的积极作用提供了新的视角。
