Marimuthu K, Gunaselvam P, Aminur Rahman M, Xavier R, Arockiaraj J, Subramanian S, Yusoff F M, Arshad A
Department of Biotechnology, AIMST University, Semeling, Bedong, Kedah Darul Aman, Malaysia.
Eur Rev Med Pharmacol Sci. 2015 May;19(10):1895-9.
Sea urchin gonad is considered as a highly prized delicacy in several countries. It is also rich in valuable bioactive compounds including polyunsaturated fatty acids (PUFAs) and β-carotene. This study was undertaken to examine the antimicrobial properties of the ovary extract from sea urchin Diadema setosum against selected Gram-negative and Gram-positive bacteria.
The ovary extract was obtained using two different solvents such as methanol and chloroform. The obtained extract was used to examine its potential antimicrobial properties against the following 11 bacterial species using the disc diffusion method: Gram-negative bacteria (Salmonella typhi, Salmonella typhimurium, Shigella flexneri, Pseudomonas aeruginosa, Aeromonas hydrophila, Acinetobacter sp, Citrobacter freundii and Klebsiella pneumonia) and Gram-positive bacteria (Bacillus subtilis, Staphylococcus epidermidis and Staphylococcus aureus). The activity was measured in terms of zone of inhibition (mm).
The methanol extract exhibited a higher zone of inhibition against all the bacteria taken for examination. Whereas, the ovary extract obtained by chloroform did not show any antimicrobial activity against S. typhi, S. epidermidis, C. freundii and K. pneumonia. The results indicated that the ovary extract obtained by methanol extracts are capable of inhibiting the growth of pathogenic microbes taken for analysis. Moreover, the result indicates the presence of antimicrobial agents in sea urchin ovary.
The study suggests that the ovary extract of D. setosum may be a potential source of antimicrobial agent for pathogenic microorganisms.
海胆性腺在多个国家被视为珍贵佳肴。它还富含包括多不饱和脂肪酸（PUFAs）和β-胡萝卜素在内的有价值的生物活性化合物。本研究旨在检测刺冠海胆卵巢提取物对选定革兰氏阴性菌和革兰氏阳性菌的抗菌特性。
使用甲醇和氯仿两种不同溶剂获得卵巢提取物。采用纸片扩散法，将所得提取物用于检测其对以下11种细菌的潜在抗菌特性：革兰氏阴性菌（伤寒沙门氏菌、鼠伤寒沙门氏菌、弗氏志贺菌、铜绿假单胞菌、嗜水气单胞菌、不动杆菌属、弗氏柠檬酸杆菌和肺炎克雷伯菌）和革兰氏阳性菌（枯草芽孢杆菌、表皮葡萄球菌和金黄色葡萄球菌）。活性以抑菌圈（毫米）来衡量。
甲醇提取物对所有检测细菌均表现出较高的抑菌圈。而用氯仿获得的卵巢提取物对伤寒沙门氏菌、表皮葡萄球菌、弗氏柠檬酸杆菌和肺炎克雷伯菌未显示任何抗菌活性。结果表明，甲醇提取物获得的卵巢提取物能够抑制所分析的致病微生物的生长。此外，结果表明海胆卵巢中存在抗菌剂。
该研究表明，刺冠海胆卵巢提取物可能是致病微生物抗菌剂的潜在来源。
