Perez-Sanchez R, Gomez-Bautista M, Grandes A E
Department of Animal Biology and Parasitology, University of Salamanca, Spain.
Ann Trop Med Parasitol. 1989 Apr;83(2):143-50. doi: 10.1080/00034983.1989.11812322.
A survey of dogs in four areas of Salamanca province (northwest Spain) was carried out to determine the prevalence of filariids in the canine population. Blood samples from 293 dogs were tested for the presence of microfilariae, using a modification of the Knott technique. For correct identification of filariid species, blood smears associated with the 43 positive Knott tests were histochemically stained for demonstration of the somatic distribution of acid phosphatase activity. The survey revealed the presence in the northwest of Spain of Dirofilaria immitis, D. repens and Dipetalonema reconditum, with an overall prevalence in Salamanca of 12.3%, 0.3% and 2.1% respectively. The greater prevalence of canine heartworm was found in the irrigated area, where 33.3% of the dogs harboured microfilariae. The relationship between the prevalence of D. immitis and mosquito populations, and the influence of host sex and age in this infection, are analysed.
为了确定犬类群体中丝状虫的流行情况，对西班牙西北部萨拉曼卡省四个地区的犬类进行了一项调查。采用改良的诺特氏技术，对293只犬的血液样本进行了微丝蚴检测。为了正确鉴定丝状虫种类，对与43次阳性诺特氏检测相关的血涂片进行了组织化学染色，以显示酸性磷酸酶活性的体细胞分布。调查显示，西班牙西北部存在犬恶丝虫、匐行恶丝虫和隐匿棘唇线虫，在萨拉曼卡的总体流行率分别为12.3%、0.3%和2.1%。在灌溉区发现犬心丝虫的流行率更高，那里33.3%的犬体内带有微丝蚴。分析了犬恶丝虫流行率与蚊虫数量之间的关系，以及宿主性别和年龄对这种感染的影响。
