Nalluri Sandeep M, Krishnan G Rajesh, Cheah Calvin, Arzumand Ayesha, Yuan Yuan, Richardson Caley A, Yang Shuying, Sarkar Debanjan
Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA.
Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA.
Mater Sci Eng C Mater Biol Appl. 2015 Sep;54:182-95. doi: 10.1016/j.msec.2015.05.043. Epub 2015 May 12.
Segmental polyurethanes exhibit biphasic morphology and can control cell fate by providing distinct matrix guided signals to increase the chondrogenic potential of mesenchymal stem cells (MSCs). Polyethylene glycol (PEG) based hydrophilic polyurethanes can deliver differential signals to MSCs through their matrix phases where hard segments are cell-interactive domains and PEG based soft segments are minimally interactive with cells. These coordinated communications can modulate cell-matrix interactions to control cell shape and size for chondrogenesis. Biphasic character and hydrophilicity of polyurethanes with gel like architecture provide a synthetic matrix conducive for chondrogenesis of MSCs, as evidenced by deposition of cartilage-associated extracellular matrix. Compared to monophasic hydrogels, presence of cell interactive domains in hydrophilic polyurethanes gels can balance cell-cell and cell-matrix interactions. These results demonstrate the correlation between lineage commitment and the changes in cell shape, cell-matrix interaction, and cell-cell adhesion during chondrogenic differentiation which is regulated by polyurethane phase morphology, and thus, represent hydrophilic polyurethanes as promising synthetic matrices for cartilage regeneration.
分段聚氨酯呈现双相形态，并且可以通过提供独特的基质引导信号来控制细胞命运，以增加间充质干细胞（MSC）的软骨形成潜力。基于聚乙二醇（PEG）的亲水性聚氨酯可以通过其基质相将不同信号传递给MSC，其中硬段是细胞相互作用域，而基于PEG的软段与细胞的相互作用最小。这些协同通讯可以调节细胞-基质相互作用，以控制软骨形成过程中的细胞形状和大小。具有凝胶状结构的聚氨酯的双相特性和亲水性提供了有利于MSC软骨形成的合成基质，软骨相关细胞外基质的沉积证明了这一点。与单相水凝胶相比，亲水性聚氨酯凝胶中细胞相互作用域的存在可以平衡细胞-细胞和细胞-基质相互作用。这些结果证明了在软骨形成分化过程中，谱系定向与细胞形状、细胞-基质相互作用和细胞-细胞粘附变化之间的相关性，这是由聚氨酯相形态调节的，因此，表明亲水性聚氨酯是用于软骨再生的有前景的合成基质。
