Vekiari S A, Gordon M H, García-Macías P, Labrinea H
National Agricultural Research Foundation, Institute of Technology of Agricultural Products, 1,S.Venizelou, Lykovrissi, GR-14123 Athens, Greece.
School of Chemistry, Food Biosciences and Pharmacy, The University of Reading, Whiteknights, P.O. Box 226, Reading RG6AP, UK.
Food Chem. 2008 Oct 15;110(4):1007-11. doi: 10.1016/j.foodchem.2008.02.005. Epub 2008 Feb 9.
Chestnuts are an important economic resource in the chestnut growing regions, not only for the fruit, but also for the wood. The content of ellagic acid (EA), a naturally occurring inhibitor of carcinogenesis, was determined in chestnut fruits and bark. EA was extracted with methanol and free ellagic acid was determined by HPLC with UV detection, both in the crude extract and after hydrolysis. The concentration of EA was generally increased after hydrolysis due to the presence of ellagitannins in the crude extract. The concentration varied between 0.71 and 21.6mgg(-1) (d.w.) in un-hydrolyzed samples, and between 2.83 and 18.4mgg(-1) (d.w.) in hydrolyzed samples. In chestnut fruits, traces of EA were present in the seed, with higher concentrations in the pellicle and pericarp. However, all fruit tissues had lower concentrations of EA than had the bark. The concentration of EA in the hydrolyzed samples showed a non-linear correlation with the concentration in the unhydrolyzed extracts.
在板栗种植区，板栗不仅是重要的水果资源，也是重要的木材资源。测定了板栗果实和树皮中天然存在的致癌抑制剂鞣花酸（EA）的含量。用甲醇提取EA，通过高效液相色谱-紫外检测法测定粗提物及水解后游离鞣花酸的含量。由于粗提物中存在鞣花单宁，水解后EA的浓度通常会增加。未水解样品中EA的浓度在0.71至21.6mg g(-1)（干重）之间，水解样品中EA的浓度在2.83至18.4mg g(-1)（干重）之间。在板栗果实中，种子中含有微量的EA，在薄膜和果皮中的浓度较高。然而，所有果实组织中EA的浓度均低于树皮。水解样品中EA的浓度与未水解提取物中EA的浓度呈非线性相关。
