Laboratory for Separation Processes and Product Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova 17, 2000 Maribor, Slovenia.
Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000 Maribor, Slovenia.
Molecules. 2020 Jun 16;25(12):2774. doi: 10.3390/molecules25122774.
The aim of the work was the optimization of the subcritical water extraction process of chestnut bark using Box-Behnken response surface methodology. The influence of process parameters, such as temperature, extraction time and solvent-solid ratio, on extraction yield, yield of the main compounds, total phenol content, total tannin content and antioxidant activity has been investigated. The identified compounds were ellagic and gallic acids, ellagitannins (vescalagin, castalagin, 1--galloyl castalagin, vescalin and castalin), sugars (maltose, glucose, fructose and arabinose) and sugar derivatives (5-HMF, furfural and levulinic acid). Finally, the optimal process conditions for obtaining the bark extract highly rich in ellagic acid and with satisfactory levels of total phenols and total tannins have been determined.
本工作旨在采用 Box-Behnken 响应面法优化板栗树皮的亚临界水提取工艺。考察了工艺参数（如温度、提取时间和溶剂固比）对提取率、主要化合物产率、总酚含量、总单宁含量和抗氧化活性的影响。鉴定出的化合物为鞣花酸和没食子酸、鞣花单宁（栗木鞣素、苛性李鞣素、1--没食子酰苛性李鞣素、栗木鞣宁和苛性李鞣宁）、糖（麦芽糖、葡萄糖、果糖和阿拉伯糖）和糖衍生物（5-HMF、糠醛和乙酰丙酸）。最后，确定了获得富含鞣花酸且总酚和总单宁含量令人满意的树皮提取物的最佳工艺条件。
