微小RNA在人脐带华通氏胶间充质干细胞神经分化中的表达变化

Altered expression of microRNAs in the neuronal differentiation of human Wharton's Jelly mesenchymal stem cells.

作者信息

Zhuang Hong, Zhang Rong, Zhang Shujie, Shu Qinmeng, Zhang Dan, Xu Gezhi

机构信息

Department of Ophthalmology, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai, China.

Research Center, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai, China.

出版信息

Neurosci Lett. 2015 Jul 23;600:69-74. doi: 10.1016/j.neulet.2015.05.061. Epub 2015 Jun 3.

DOI:10.1016/j.neulet.2015.05.061

PMID:26049006

Abstract

Mesenchymal stem cells (MSCs) have the capacity to generate multiple tissues of mesodermal origin, and also have the potential to trans-differentiate into neurons. We isolated MSCs from the Wharton's jelly of the human umbilical cord (WJ-MSCs), and efficiently induced WJ-MSCs into neuron-like cells using a modified method. After neuronal induction for 12 days, most of WJ-MSCs expressed mature neuronal marker MAP2 (83 ± 7%), and meanwhile some adopted neuronal morphology. WJ-MSCs also expressed Nestin (34 ± 6%), NSE (30 ± 5%), and GFAP (12 ± 3%). Moreover, we used miRNA microarray to analyze the differentially expressed miRNAs in neuronal differentiation of WJ-MSCs. Microarray analysis revealed discrepant miRNA profiles in the uninduced WJ-MSCs and WJ-MSCs derived neurons. Six miRNAs were chosen for further qRT-PCR validation. Among these 6 miRNAs, four miRNAs (miR-1290, miR-26b, miR-194, and miR-124a) were up-regulated and 2 miRNAs (miR-4521 and miR-543) were down-regulated in the WJ-MSCs derived neurons. In conclusion, WJ-MSCs could be efficiently induced into neuron-like cells. More importantly, our findings suggested that miRNAs might play important roles in the neuronal differentiation of WJ-MSCs.

摘要

间充质干细胞（MSCs）具有生成多种中胚层来源组织的能力，并且也有转分化为神经元的潜力。我们从人脐带华通氏胶中分离出MSCs（WJ-MSCs），并使用改良方法将WJ-MSCs高效诱导为神经元样细胞。神经元诱导12天后，大多数WJ-MSCs表达成熟神经元标志物MAP2（83±7%），同时一些呈现神经元形态。WJ-MSCs还表达巢蛋白（Nestin，34±6%）、神经元特异性烯醇化酶（NSE，30±5%）和胶质纤维酸性蛋白（GFAP，12±3%）。此外，我们使用miRNA微阵列分析WJ-MSCs神经元分化过程中差异表达的miRNA。微阵列分析揭示了未诱导的WJ-MSCs和源自WJ-MSCs的神经元中不同的miRNA谱。选择6种miRNA进行进一步的qRT-PCR验证。在这6种miRNA中，4种miRNA（miR-1290、miR-26b、miR-194和miR-124a）在源自WJ-MSCs的神经元中上调，2种miRNA（miR-4521和miR-543）下调。总之，WJ-MSCs可以被高效诱导为神经元样细胞。更重要的是，我们的研究结果表明miRNA可能在WJ-MSCs的神经元分化中发挥重要作用。