Liu Can, Sheng Jiping, Chen Lin, Zheng Yanyan, Lee David Yue Wei, Yang Yang, Xu Mingshuang, Shen Lin
†College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, China.
§Bioorganic and Natural Products Laboratory, McLean Hospital, Harvard Medical School, 115 Mill Street, Belmont, Massachusetts 02478, United States.
J Agric Food Chem. 2015 Jul 8;63(26):6009-18. doi: 10.1021/acs.jafc.5b02218. Epub 2015 Jun 22.
Bacillus subtilis strain B154, isolated from Agaricus bisporus mushroom compost infected by red bread mold, exhibited antagonistic activities against Neurospora sitophila. Antifungal activity against phytopathogenic fungi was also observed. The maximum antifungal activity was reached during the stationary phase. This antifungal activity was stable over a wide pH and temperature range and was not affected by proteases. Assay of antifungal activity in vitro indicated that a purified antifungal substance could strongly inhibit mycelia growth and spore germination of N. sitophila. In addition, treatment with strain B154 in A. bisporus mushroom compost infected with N. sitophila significantly increased the yield of bisporus mushrooms. Ultraviolet scan spectroscopy, tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis, matrix-associated laser desorption ionization time-of-flight mass spectrometry, and electrospray ionization tandem mass spectrometry analyses revealed a molecular weight consistent with 1498.7633 Da. The antifungal compound might belong to a new type of lipopeptide fengycin.
从被红面包霉感染的双孢蘑菇堆肥中分离出的枯草芽孢杆菌菌株B154，对嗜神经孢子菌表现出拮抗活性。还观察到对植物病原真菌的抗真菌活性。在稳定期达到最大抗真菌活性。这种抗真菌活性在很宽的pH和温度范围内都很稳定，且不受蛋白酶影响。体外抗真菌活性测定表明，一种纯化的抗真菌物质能强烈抑制嗜神经孢子菌的菌丝生长和孢子萌发。此外，用菌株B154处理被嗜神经孢子菌感染的双孢蘑菇堆肥，显著提高了双孢蘑菇的产量。紫外扫描光谱、十二烷基肌氨酸钠-聚丙烯酰胺凝胶电泳、基质辅助激光解吸电离飞行时间质谱和电喷雾电离串联质谱分析显示，分子量与1498.7633 Da一致。该抗真菌化合物可能属于一种新型脂肽丰原素。
