Song Xue-Ge, Han Ming-Hui, He Feng, Wang Su-Yan, Li Chao-Hui, Wu Gui-Chun, Huang Zi-Gang, Liu Dong, Liu Feng-Quan, Laborda Pedro, Shi Xin-Chi
School of Life Sciences, Nantong University, Nantong, China.
College of Life Science, Anhui Normal University, Wuhu, China.
Front Microbiol. 2020 May 20;11:958. doi: 10.3389/fmicb.2020.00958. eCollection 2020.
is a fatal canker pathogen that causes significant reduction of crop yield in pear orchards. invades the trunk phloem, and is difficult to control by chemical treatment. In this work, it was found for the first time that -produced dipicolinic acid (DPA) exhibits antifungal activity against different canker pathogens, including , , , and . Growth inhibition of was observed at less than 5 mM concentration (pH = 5.6). DPA showed the highest antifungal activity at acidic pH values and in the presence of bivalent metals, such as zinc(II), cobalt(II), and copper(II). Measurement of mRNA expression levels and scanning electron microscope (SEM) observations revealed that DPA causes apoptosis via inhibition of chitin biosynthesis and subsequent cell lysis. Interestingly, DPA showed high stability in the pear bark and was able to cross the pear tree bark into the phloem, protecting the internal phases of the pear trunk. In preventive applications, DPA reduced the canker symptoms of on Cuigan pear trees by 90%. Taken together, an efficient strategy for the management of -caused canker disease was developed using a novel antifungal agent, DPA, with strong antifungal activity and particular diffusion properties.
是一种致命的溃疡病菌，会导致梨园作物产量大幅下降。它侵入树干韧皮部，难以通过化学处理进行控制。在这项工作中，首次发现产生的吡啶二羧酸（DPA）对包括、、和在内的不同溃疡病菌具有抗真菌活性。在浓度低于5 mM（pH = 5.6）时观察到对的生长抑制。DPA在酸性pH值以及存在二价金属（如锌（II）、钴（II）和铜（II））的情况下表现出最高的抗真菌活性。mRNA表达水平的测量和扫描电子显微镜（SEM）观察表明，DPA通过抑制几丁质生物合成和随后的细胞裂解导致细胞凋亡。有趣的是，DPA在梨树皮中表现出高稳定性，并且能够穿过梨树树皮进入韧皮部，保护梨树干的内部组织。在预防性应用中，DPA使翠冠梨树上由引起的溃疡症状减少了90%。综上所述，利用具有强大抗真菌活性和特殊扩散特性的新型抗真菌剂DPA，开发了一种有效的防治由引起的溃疡病的策略。
